›› 2020, Vol. 40 ›› Issue (2): 180-.doi: 10.3969/j.issn.1674-8115.2020.02.006

• Original article (Basic research) • Previous Articles     Next Articles

Inhibition of resveratrol on colonic movement in mice and its mechanism

YANG Meng, LU Hong-li, HUANG Xu, XU Wen-xie   

  1. 1. Department of Physiology, Medicine College, Jingchu University of Technology, Jingmen 448000, China; 2. Department of Anatomy and Physiology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200240, China
  • Online:2020-02-28 Published:2020-03-20
  • Supported by:
    General Program of National Natural Science Foundation of China (31571180).

Abstract: Objective · To investigate the effect of resveratrol (Res) on colonic movement in mice and its mechanism. Methods · The effects of Res (10, 20, 40, and 80 μmol/L) on spontaneous contraction of colonic smooth muscle in mice and colonic migrating motor complex (CMMC) were recordedusing a multi-channel physiological recorder. Colonic smooth muscle strips were pretreated with the following tool medicines: BayK8644 (an activator of L-type Ca2+ channel), TEA (tetraethylammonium, a non-selective K+ channel blocker), TTX (tetrodotoxin, a Na+ channel blocker), atropine (a cholinergic neuron inhibitor), L-NAME [Nω-nitro-L-arginine methyl ester, a nitric oxide (NO) synthase inhibitor], NPPB[5-nitro-2-(3-phenylpropylamino) benzoic acid, an ANO1 (anoctamin 1) channel inhibitor] and apamin [a small conductance Ca2+-activated K+ channel 3 (SK3) channel inhibitor].elution, the effects of Res on spontaneous contraction of colonic smooth muscle strips before and after pretreatment were recorded and compared. Finally, the effect of Res on L-type Ca2+ channel currents were recordedusing the whole-cell patch clamp technique in freshly dispersed colonic smooth muscle cells. Results · Res significantly inhibited colonic smooth muscle spontaneous contraction in a dose-dependent manner and markedly decreased CMMC in the proximal and the distal colon. BayK8644 significantly blocked the Res-induced inhibition. Besides, Res dramatically inhibited L-type Ca2+ channel currents in colonic smooth muscle cells. However, TEA did not block the inhibitory effect of Res. In addition, TTX, atropine, L-NAME, NPPB and apamin had no effect on the inhibitory effect of Res on spontaneous contraction of colonic smooth muscle. Conclusion · Res inhibits colonic movement via inhibiting L-type Ca2+ channel in mice, and its mechanism may be irrelevant to potassium channel, the enteric nervous system, NO and interstitial cell.

Key words: resveratrol (Res), colonic movement, L-type Ca2+ channel, mice