›› 2020, Vol. 40 ›› Issue (4): 443-.doi: 10.3969/j.issn.1674-8115.2020.04.005
• Original article (Basic research) •
GE Kun, QU Chun, JIA Wei, ZHAO Ai-hua
Objective · To develop a quantitative method of 7α-hydroxy-4-cholesten-3-one (C4), cholic acid (CA) and chenodeoxycholic acid (CDCA) in human plasma. Methods · After extraction of C4, CA and CDCA with acetonitrile plasma, they were quantified with standard curve correctedthe internal standards based on Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Results · The limits of detection (LOD) of C4, CA and CDCA were 0.16, 0.02 and 0.04 nmol/L respectively; All three metabolites had good linear relationships (correlation coefficients R2 were over than 0.998). The relative standard deviations (RSDs) of repeatabilities were below 3.0%. The RSDs of inter-day and intra-day precision were less than 6%, and the RSDs of stabilities at 4 °C were below 10% within 7 days. The average added recoveries of C4, CA and CDCA were 97.7%, 113.3% and 105.0%, respectively. Conclusion · This method is of high detective sensitivity, good precision and stability, which meets the quantitative requirements of plasma biological samples.
cholic acid (CA),
chenodeoxycholic acid (CDCA),
GE Kun, QU Chun, JIA Wei, ZHAO Ai-hua. Quantification of human plasma 7α-hydroxy-4-cholesten-3-one and bile acidsUPLC-MS/MS[J]. , 2020, 40(4): 443-.
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