Acetylation involved in bacterial susceptibility to ribosome-targeting antibiotics

doi:10.3969/j.issn.1674-8115.2021.03.004

Abstract

Abstract: Objective

·To investigate the effects of acetylation on the bacterial susceptibility to ribosome-targeting antibiotics.

Methods

·The minimum inhibitory concentrations (MICs) of puromycin, paromomycin, tetracycline and spectinomycin for Salmonella enterica serovar Typhimurium (S. Typhimurium) 14028S were determined by the broth dilution method. The solid mediums with concentration lower than MIC was prepared. The spot dilution assay was employed to determine the susceptibility of S. Typhimuriumstrain 14028S, protein acetyltransferase (pat) knockout strain (Δpat), NAD⁺-dependent protein deacylase (cobB) knockout strain (ΔcobB) and acetate kinase (ackA) knockout strain (ΔackA) to four kinds of ribosome-targeting antibiotics.

Results

·Increase of acetylation (ΔackA) promoted the susceptibility of S. Typhimurium to puromycin, but promoted the resistance of S. Typhimurium to paromomycin. However, the sensitivity of S. Typhimurium to tetracycline and spectinomycin was not affected by the increase of acetylation (ΔackA or ΔcobB) or decrease of acetylation (Δpat).

Conclusion

·Acetylation is involved in the susceptibility of S. Typhimurium to ribosome-targeting antibiotics, suggesting that acetylation of ribosomal proteins may contribute to bacterial antibiotic sensitivity.

Key words: acetylation, ribosome, ribosome-targeting antibiotics

CLC Number:

Ya-nan LAI, Yu-feng YAO, Xiao-kui GUO, Jin-jing NI. Acetylation involved in bacterial susceptibility to ribosome-targeting antibiotics[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(3): 308-313.

Figures/Tables 6

TrendMD

References 22

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Stage	Concentration/(mg·L^-1)	Source	Antibiotic volume/mL	LB medium volume/mL	Concentration obtained/(mg·L^-1)	Concentration in test/(mg·L^-1)
1	1 280	Stock	1	9	128	64
2	128	Stage 1	1	1	64	32
3	128	Stage 1	1	3	32	16
4	128	Stage 1	1	7	16	8
5	16	Stage 4	1	1	8	4
6	16	Stage 4	1	3	4	2
7	16	Stage 4	1	7	2	1
8	2	Stage 7	1	1	1	0.5
9	2	Stage 7	1	3	0.5	0.25
10	2	Stage 7	1	7	0.25	0.125
11	0.25	Stage 10	1	1	0.125	0.062 5
12	0.25	Stage 10	1	3	0.062 5	0.031 25

Stage	Concentration/(mg·L^-1)	Source	Antibiotic volume/mL	LB medium volume/mL	Concentration obtained/(mg·L^-1)	Concentration in test/(mg·L^-1)
1	1 280	Stock	1	9	128	64
2	128	Stage 1	1	1	64	32
3	128	Stage 1	1	3	32	16
4	128	Stage 1	1	7	16	8
5	16	Stage 4	1	1	8	4
6	16	Stage 4	1	3	4	2
7	16	Stage 4	1	7	2	1
8	2	Stage 7	1	1	1	0.5
9	2	Stage 7	1	3	0.5	0.25
10	2	Stage 7	1	7	0.25	0.125
11	0.25	Stage 10	1	1	0.125	0.062 5
12	0.25	Stage 10	1	3	0.062 5	0.031 25

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