Effects of knockdown of immunoregulatory protein B7-H3 gene on malignant proliferation, invasion and stem cell-like characteristics of bladder cancer cells

doi:10.3969/j.issn.1674-8115.2021.11.008

JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2021, Vol. 41 ›› Issue (11): 1454-1460.doi: 10.3969/j.issn.1674-8115.2021.11.008

• Basic research • Previous Articles Next Articles

Effects of knockdown of immunoregulatory protein B7-H3 gene on malignant proliferation, invasion and stem cell-like characteristics of bladder cancer cells

Xiao-han WANG¹(), Yun-lin YE², Kang-hua XIAO², Zhi-xin ZHAO³, Jing-ya WU⁴

^1.Department of surgery, Henan Vocational College of nursing, Anyang 455000, China
^2.Department of Urology and oncology, Cancer Hospital Affiliated to Zhongshan University, Guangzhou 510030, China
^3.Pharmacy teaching and Research Office, Henan Vocational College of nursing, Anyang 455000, China
^4.Department of traditional Chinese medicine, Henan Province Anyang District Hospital, Anyang 455000, China

Online:2021-11-28 Published:2021-12-03
Contact: Xiao-han WANG E-mail:wang136473@163.com
Supported by:
2018 Henan Province Medical Science and Technology Tackling Project(2018020950)

Abstract

Abstract: Objective

·To investigate the effect of gene interfering with immune regulatory protein B7-H3 on malignant proliferation and stemness characteristics of bladder cancer J82 cells.

Methods

·Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect shRNA interference efficiency. The proliferation of J82 cells was detected by clone formation assay. Cell apoptosis was detected by flow cytometry. Cell invasion was detected using Transwell asssay. The tumor-cloning ability was observed using tumor sphere formation experiment. Apoptosis, invasion, and expression and stemness-related proteins were detected by Western blotting.

Results

·B7-H3 is highly expressed in bladder cancer tissues and bladder cancer cell lines (P=0.000). shRNA2 was identified as the most efficient interference with B7-H3 gene in three shRNAs (P=0.000). The interference of B7-H3 inhibited the proliferation, invasion, tumor cloning ability and stem cell-like characteristics of J82 cells (P=0.000). Furthermore, B7-H3 interference promoted the apoptosis of J82 cells (P=0.000).

Conclusion

·The gene interference of immunoregulatory protein B7-H3 can inhibit the malignant proliferation, invasion and stemness characteristics of bladder cancer J82 cells, providing a new possibility and effective evidence for the treatment of bladder cancer.

Key words: B7-H3, Bladder cancer, J82 cells, Stemness characteristics

CLC Number:

R737.14

Xiao-han WANG, Yun-lin YE, Kang-hua XIAO, Zhi-xin ZHAO, Jing-ya WU. Effects of knockdown of immunoregulatory protein B7-H3 gene on malignant proliferation, invasion and stem cell-like characteristics of bladder cancer cells[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(11): 1454-1460.

Figures/Tables 6

Fig 1 Expression of B7-H3 in bladder cancer tissues and bladder cancer cell lines

Fig 2 Detection of B7-H3 interference efficiency of bladder cancer cells J82 transfected with the three shRNAs

Fig 3 Detection of J82 cells proliferation

Fig 4 Detection of apoptosis of J82 cells

Fig 5 Invasion of J82 cells

Fig 6 Stemness characteristics of J82 cells

TrendMD

References 21

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Effects of knockdown of immunoregulatory protein B7-H3 gene on malignant proliferation, invasion and stem cell-like characteristics of bladder cancer cells

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