Effect of intragastric treatment of sodium cromoglycate on dextran sulfate sodium-induced ulcerative colitis in BALB/c mice

doi:10.3969/j.issn.1674-8115.2022.10.002

Abstract

Abstract:

Objective ·To explore the role of mast cell membrane stabilizer sodium cromoglycate (Crom) in the modeling of ulcerative colitis (UC) in mice. Methods ·Mice were divided into 3 groups, i.e.control group (n=5), dextran sulfate sodium (DSS) group (n=5) and DSS+Crom group (n=5). Mice in the control group drank water for 7 d, and mice in the DSS group drank 3% DSS for 7 d to establish UC model. Meanwhile, mice in the DSS+Crom group were treated with Crom since the 3^rd day during the establishment of UC. The changes of body mass and disease activity index (DAI) scores among the three groups were compared. Hematoxylin-eosin staining (H-E staining) and toluidine blue staining were used to observe the histopathology injury and the number of mast cells in colon tissues. Flow cytometry was used to detect the immune cell infiltration of spleen. Differences among the three groups were compared by using one-way ANOVA. Least significant difference (LSD) was used to further compare the differences between each two groups. Results ·Compared with the control group, the mice in the DSS group had severe hematochezia and intestinal barrier injury, the body weight of the mice in the DSS group decreased significantly (P=0.005), the DAI score increased significantly (P=0.001), the colon length shortened significantly (P=0.003), and toluidine blue staining showed that the number of mast cells in the intestine increased (P=0.000). Moreover, compared with the DSS group, the blood in the stool of the mice in the DSS+Crom group decreased, and the colon length increased (P=0.013); the intestinal pathology showed that the inflammation was improved, and the number of mast cells was reduced (P=0.000). Conclusion ·Stabilizing mast cells with sodium cromoglycate could effectively reduce colon inflammation of mice in UC, which indirectly indicates that mast cells participate in the pathogenesis of UC.

Key words: BALB/c mice, ulcerative colitis (UC), sodium cromoglycate (Crom), mast cell

CLC Number:

Q95-33

GE Yiqin, HUANG Yuji, LI Weize, LI Yanning, LI Li. Effect of intragastric treatment of sodium cromoglycate on dextran sulfate sodium-induced ulcerative colitis in BALB/c mice[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2022, 42(10): 1375-1382.

Figures/Tables 7

TrendMD

References 28

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Score	Weight loss/%	Stool consistency	Bloody
0	0	Normal	-
1	≤5	Loose	+
2	≤10	Soft， but shaped	++
3	≤20	Loose stools	+++
4	>20	Liquid	Blood to the naked eye

Score	Weight loss/%	Stool consistency	Bloody
0	0	Normal	-
1	≤5	Loose	+
2	≤10	Soft， but shaped	++
3	≤20	Loose stools	+++
4	>20	Liquid	Blood to the naked eye

Score	Epithelial cell structure	Inflammatory cell infiltration	Goblet cell count	Areas of inflammatory cell infiltration
0	Normal	Normal	Normal	No
1	Slight loss	Locally， superficial	Part of the loss	Local
2	The crypt is severely deformed	Diffuse， base	The number of missing is over 50%	More than 50%
3	Crypt disappear	Full thickness	No	More than 75%

Score	Epithelial cell structure	Inflammatory cell infiltration	Goblet cell count	Areas of inflammatory cell infiltration
0	Normal	Normal	Normal	No
1	Slight loss	Locally， superficial	Part of the loss	Local
2	The crypt is severely deformed	Diffuse， base	The number of missing is over 50%	More than 50%
3	Crypt disappear	Full thickness	No	More than 75%

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