Journal of Shanghai Jiao Tong University (Medical Science) ›› 2022, Vol. 42 ›› Issue (10): 1458-1465.doi: 10.3969/j.issn.1674-8115.2022.10.012

• Review • Previous Articles    

Advances in single-cell RNA sequencing in glomerular diseases

XING Haifan(), FAN Ying()   

  1. Department of Nephrology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China
  • Received:2022-05-09 Accepted:2022-09-19 Online:2022-10-28 Published:2022-12-02
  • Contact: FAN Ying;
  • Supported by:
    National Natural Science Foundation of China(81870468);Foundation of National Facility for Translational Medicine (Shanghai) of Shanghai Jiao Tong University(TMSK-2021-109)


Glomerular diseases are a leading cause of chronic kidney disease and end-stage renal failure. Understanding the molecular mechanism underlying the initiation and progression of glomerular diseases is important to early identify risk factors for disease development and rescue renal function. The kidney is composed of complex and highly differentiated cells, which is a major challenge in the study of glomerular diseases. Bulk RNA sequencing cannot assess differential transcription or the interactions between cell types. Single-cell RNA sequencing (scRNA-seq) technology has developed rapidly in recent years, making it possible to study a wide range of cell types in kidney, urine and blood. The application of scRNA-seq to glomerular diseases helps to construct a comprehensive cell atlas, elucidate complex cellular and molecular mechanisms, develop efficient and accurate prognostic biomarkers and cell-specific therapies. This review focuses on the latest research advances in scRNA-seq in primary and secondary glomerular diseases.

Key words: single-cell RNA sequencing (scRNA-seq), transcriptome, glomerulonephritis, diabetic nephropathies, lupus nephritis, glomerulonephritis, IGA, glomerulosclerosis, focal segmental, glomerulonephritis, membranous

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