›› 2009, Vol. 29 ›› Issue (8): 909-.

• Original article (Basic research) • Previous Articles     Next Articles

Effects of hypoxia on expression of Cyr61/CCN1 in murine osteoblasts

CUI Lun, WANG Jun, QI Jin, ZHU Ya-ping, XU Fu-ping, WEI Li, ZHANG Feng-hua, DENG Lian-fu   

  1. Shanghai Institute of Traumatology and Orthopaedics, Department of Orthopedics, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Online:2009-08-25 Published:2009-09-27
  • Supported by:

    National Natural Science Foundation of China, 30672145; Shanghai Innovation Project, 07dz22008

Abstract:

Objective To investigate the effects of hypoxia on the expression of cysteine-rich protein 61(Cyr61/CCN1) in murine osteoblasts. Methods Murine osteoblasts were cultured under oxygen of lower concentration or treated with desferrioxamine, and Real-time PCR and Western blotting were employed to detect the expression of HIF-1α, Cyr61/CCN1 and vascular endothelial growth factor in osteoblasts at different time points. HIF-1α gene or VHL gene were knocked out in murine osteoblasts, and the expression of Cyr61/CCN1 in murine osteoblasts was detected. Results HIF-1α pathway was activated in murine osteoblasts under the condition of hypoxia, and the expression of Cyr61/CCN1 in osteoblasts was significantly increased (P<0.05 or P<0.01). The expression of Cyr61/CCN1 was significantly decreased in murine osteoblasts with HIF-1α gene knockout, and that was significantly increased in osteoblasts with VHL gene knockout (P<0.05 or P<0.01). Conclusion The expression of Cyr61/CCN1 in murine osteoblasts may be up-regulated by hypoxia through activation of HIF-1α pathway.

Key words: hypoxia, hypoxia inducible factor-1α, osteoblasts, Cyr61/CCN1, vascular endothelial growth factor

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