›› 2009, Vol. 29 ›› Issue (12): 1459-.

• Original article (Basic research) • Previous Articles     Next Articles

Effects of different doses of topiramate on expression of NCAM and GAP-43 mRNA in hippocampus of rats with epilepsy

WU Dan-hong1, ZHONG Ping2, BAO Guan-shui1, ZHOU Hong1, SHEN Jun1, HUANG Fei-fei1   

  1. 1. Department of Neurology, The Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, China；2. Department of Neurology, Shanghai Traditional Chinese Medical Integrated Hospital, Shanghai 200083, China
  • Online:2009-12-25 Published:2009-12-25
  • Supported by:

    Foundation for Excellent Young Higher Education Teachers of Shanghai, 07JWYQ02

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Abstract:

Objective To explore the effects of different doses of topiramate (TPM) on the expression of nerve cell adhesion molecule (NCAM) and growth-associated protein 43 (GAP-43) mRNA in hippocampus of rats with epilepsy. Methods Forty-eight rats were randomly divided into normal control group, kainic acid (KA) group, 10 mg/kg TPM group, 40 mg/kg TPM group, 100 mg/kg TPM group and 400 mg/kg TPM group (n=8). The models of rats with epilepsy treated by different doses of TPM were established. The behavior of rats was observed, and the expression of NCAM and GAP-43 mRNA in hippocampus of rats was determined by Real-time PCR. Results The expression of NCAM and GAP-43 mRNA in KA group was significantly higher than that in normal control group (P<0.01), while there was no significant difference between 10 and 40 mg/kg TPM groups and KA group, that in 100 and 400 mg/kg TPM groups was significantly lower than that in KA group (P<0.01), and that in 400 mg/kg TPM group was significantly lower than that in 100 mg/kg TPM group (P<0.01). Conclusion KA can up-regulate the expression of NCAM and GAP-43 mRNA in hippocampus of rats with epilepsy. Higher dose of TPM can inhibit the expression of NCAM and GAP-43 mRNA, and the inhibitory effect is related with the dose of TPM.

Key words: epilepsy, topiramate, nerve cell adhesion molecule, growth-associated protein 43