›› 2012, Vol. 32 ›› Issue (10): 1307-.doi: 10.3969/j.issn.1674-8115.2012.10.006

• Original article (Basic research) • Previous Articles     Next Articles

Design, preparation and synchronous transplantation experiments of laser micropore porcine acellular dermal matrix

ZENG Tao-fang1, LUO Xu2, XIN Guo-hua3, WAN Li4, XU Jian-jun2, XIA Wei-dong2, MAO Cong5, WANG Ping6, LIN Cai2,5   

  1. 1.Department of Burn, the People´s Hospital of Yichun, Yichun 336000, China；2.Department of Burn, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China；3.Department of Burn, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China； 4.Department of Pathology, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China；5.College of Material Science and Engineering, South China University of Technology, Guangzhou 510641, China；6.Shanghai Institute of Technical Physics of the Chinese Academy of Sciences, Shanghai 200083, China
  • Online:2012-10-28 Published:2012-11-05
  • Supported by:

    Natural Science Foundation of Zhejiang Province, Z2080985, Y407241；Wenzhou Science and Technology Project, Y20080101；Lucheng District Science and Technology Project, S070109

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Abstract:

Objective To prepare laser micropore porcine acellular dermal matrix (LPADM), a new type of dermal substitute, and to verify the healing effect on full-thickness skin defect. Methods Splitthickness dermis of the healthy white pigs was harvested and treated by high-sodium-SDS method, and the porcine acellular dermal matrix (ADM) was obtained. Besides, LPADM was prepared using laser micropore technology on the dermis, followed by the procedure of high-sodium-SDS acellular treatment. The physical properties of LPADM were evaluated. Four full-thickness skin wounds (2.0 cm×2.0cm) were created on each dorsum of 30 SD rats. Group L (LPADM grafted with the split-thickness autograft), group A (nonporous ADM grafted with the split-thickness autograft), group F (full-thickness autograft) and group C (split-thickness autograft) were divided on the basis of different grafts used through one-step method. Six rats were randomly selected on day 3, 5, 7, 10 and 14 after surgery, the survival of skin graft was observed, and histological examination was conducted after sacrifice. Results The well-prepared micropore LPADM was characterized by its porcelain white color, softness and good elasticity. Histological examination revealed that LPADM was totally devoid of epidermis and cellular components. On day 3 after surgery, the cavity-like structure formed by surrounding endothelial cells was observed in group L. On day 3, 5, 7 and 10 after surgery, the grafts of group L and C survived well, and new vessels were generated in group L. However, epidermal necrosis with black and blistering tissues occurred in group A, while some grafts blistering with ruddy epidermal basement were observed in group F. On day 14 after surgery, abundant blood vessels formed in group L, and all the grafts survived in group L, C and F, with the wound healing rates of (99.10±0.66)%, (99.25±0.23)%, and (97.07±1.32)%, respectively, and there was no significant difference among groups (P>0.05). The wound healing rate of group A was (27.46±2.05)%, which was significantly lower than those of the other groups (P<0.01). Conclusion The new type of micropore porcine acellular dermis matrix can be used as an ideal dermal substitute due to high survival rate when grafted with the split-thickness autograft and the ability of initiating early revascularization.

Key words: dermal substitutes, acellular dermal matrix, laser, microporous, transplantation