Objective To observe the clinical effects of different desensitizers on cervical dentin hypersensitivity. Methods Seventy-six patients (214 teeth) with cervical dentin hypersensitivity were divided into moderate symptom group (n=110) and severe symptom group (n=104) according to the severity of hypersensitivity. Desensitization was randomly performed with Clinpro TMXT Varnish, Gluma and 75% sodium fluoride glycerol in each group. The efficacy was evaluated immediately after treatment and 1 month, 3 months and 6 months after treatment, and the effective rates were calculated. Results There was no significant difference in the effective rates immediately after treatment and 1 month, 3 months and 6 months after treatment between moderate symptom group and severe symptom group (P>0.05). The efficacy of Clinpro TMXT Varnish and Gluma was better than that of sodium fluoride glycerol immediately after treatment within the same group (P<0.05). The efficacy decreased with time for all the three desensitizers. Six months after treatment, the effective rate of Clinpro TMXT Varnish was higher than those of Gluma and 75% sodium fluoride glycerol (P<0.05). Conclusion All the three desensitizers may play a role in the treatment of cervical dentin hypersensitivity, and Clinpro TMXT Varnish may yield better long-term effects.