Original article (Basic research)

Effects of trichosanthin on apoptosis and proliferation of colorectal cancer cell line CMT-93

  • ZHOU Lin ,
  • LIU Fei ,
  • ZHOU Guang-yan ,
  • et al
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  • Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Online published: 2014-04-02

Supported by

National Natural Science Foundation of China, 31370904, 30972691; New Talents Training Plan of Shanghai's Health System, 2011XYQ015; Major Fundamental Research Program of Science and Technology Commission of Shanghai Municipality,11JC1410802

Abstract

Objective To investigate effects of trichosanthin (TCS) on apoptosis and proliferation of colorectal cancer (CRC) cell line CMT-93. Methods CMT-93, a mouse colorectal cell line cultured in vitro, was treated by TCS of different mass concentrations. The control group was CMT-93 without being treated by TCS. The activity of cell proliferation was measured by the Real-Time Cellular Analysis (RTCA) system. The proliferation and apoptosis was detected by the flow cytometry. Real-Time PCR was used to evaluate gene expressions related to the apoptosis. Expressions of Akt and γ-H2AX and the phosphorylation level of Akt (pAkt 473 and pAkt 308)  were assayed by the Western blotting. Results Compared to the control group, TCS of 5.0 μg/mL significantly inhibited the cell viability of CMT-93 cells and induced apoptosis of CMT-93 cells. The mRNA levels of Bid, Bax, and Bad were up-regulated in TCS-treated group. The expressions of pAkt 473 and pAkt 308 decreased significantly after treated by TCS, while the expression of γ-H2AX increased. Conclusion The results suggest that TCS can induce the apoptosis and inhibit the proliferation of CMT-93 cells. The mechanism may be relevant to inhibiting the expressions of active proapoptotic related proteins of Akt and DNA damage of the TCS.

Cite this article

ZHOU Lin , LIU Fei , ZHOU Guang-yan , et al . Effects of trichosanthin on apoptosis and proliferation of colorectal cancer cell line CMT-93[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2014 , 34(3) : 257 . DOI: 10.3969/j.issn.1674-8115.2014.03.001

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