Objective To explore the effects of different cryopreservation carriers on vitrification cryopreservation of human embryos. Methods Human embryos of D3 were vitrification cryopreserved using different cryopreservation carriers (265 half-closing cryopreservation carriers and 136 closing cryopreservation carriers). Pregnant outcomes were compared between the two groups. Results The survival rate, the clinical pregnancy rate, and the implantation rate of 258 (7 cancelled) halfclosing cryopreservation carriers were 96.88%, 48.44%, and 32.20%, respectively. Whereas those rates of 132 (4 cancelled) closing cryopreservation carriers were 95.31%, 47.72%, and 32.28%. The differences of mean age, years, types and causes of infertility, ratio of in vitro fetilization or intracytoplasmic sperm injection (IVF/ICSI), embryo recovery rate and transfer number, rate of pregnancy, and implant rate between two groups were not statistically significant (P>0.05). Conclusion The closing cryopreservation carrier was as effective as the half-closing cryopreservation carrier and can protect embryos from crossing contamination of pathogens. It is an ideal method to preserve human embryos.