Objective To investigate the role of chemokine CCL19 in colorectal carcinoma tissues. Methods SW620 Cell line with highest expression level of CCL19 was screened for the study by the Real-Time quantitative PCR and Western blot. CCL19 of SW620 was silenced by siRNA. The changes of proliferation, migration and invasion, and proangiopoiesis abilities of SW620 were detected by the CCK-8, Transwell, and tube formation assay, respectively. Results The Western blotting and Real-Time PCR confirmed that the expression of CCL19 in SW620 was higher than that of other cell lines. The proliferation, migration, invasion, and proangiopoiesis abilities of SW620 cells within 48-120 h were significantly improved after CCL19 being silenced. Conclusion The expression level of CCL19 in SW620 is relatively high. Silencing CCL19 can significantly enhance the proliferation, migration, invasion, and proangiopoiesis abilities of SW620 cells in vitro, so it is believed that the CCL19 can inhibit the development of colorectal cancer and is potential to be applied to the research of anticancer drugs.