Original article (Basic research)

Effects of silencing TGFβ1 on growth and proliferation of hepatic stellate cell line T6

  • ZHANG Rong-hua ,
  • YAN Guo-he ,
  • WANG Guo-jian ,
  • et al
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  • 1.Department of Integrated Chinese and Western Medicine, Southwest Hospital, the Third Military Medical University of Chinese PLA, Chongqing 400038, China; 2.State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Military Preventive Medicine, the Third Military Medical University of Chinese PLA, Chongqing 400038, China

Online published: 2014-09-26

Supported by

National Natural Science Foundation of China,81273918;Project of Traditional Chinese Medicine Science and technology of Chongqing,2012-2-63;Specific Project of Traditional Chinese Medicine of Chinese PLA,2010ZYZ231

Abstract

Objective To observe the effects of silencing transforming growth factor β1 (TGFβ1) on the growth and proliferation of hepatic stellate cell line T6 (HSC-T6). Methods The TGFβ1 shRNA lentiviral vector particles with high infectivity were transfected into HSC-T6 cells. The controls were HSC-T6 cells that were not infected or infected by empty virus. The expression of green fluorescent protein (GFP) of the HSC-T6 cells was observed under the inverted fluorescence microscope. The effects of TGFβ1 shRNA lentivirus on the growth and proliferation of HSC-T6 cells were detected by the CCK-8. The expressions of the gene and encoding protein of TGFβ1 and proliferating cell nuclear antigen (PCNA) of HSC-T6 cells were detected by the RT-PCR and Western blotting, respectively. The quantitative analysis was then conducted. Results HSC-T6 cells transfected by TGFβ1 shRNA lentivirus significantly expressed GFP. The results of CCK-8 showed that the growth and proliferation of HSC-T6 cells transfected by TGFβ1 shRNA lentivirus were slower than those of controls and the differences were statistically significant after being cultured for 48 h (P<0.01). The results of RT-PCR and Western blotting revealed that the TGFβ1 shRNA lentivirus effectively silenced the TGFβ1 and down-regulated the expressions of gene and encoding protein of PCNA of HSC-T6 cells. The differences of transfected cells and controls were statistically significant (P<0.01). Conclusion Silencing TGFβ1 can effectively inhibit the growth and proliferation of HSC-T6 cells and down-regulate the expression of PCNA.

Cite this article

ZHANG Rong-hua , YAN Guo-he , WANG Guo-jian , et al . Effects of silencing TGFβ1 on growth and proliferation of hepatic stellate cell line T6[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2014 , 34(9) : 1315 . DOI: 10.3969/j.issn.1674-8115.2014.09.012

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