Objective To detect the expressions in Notch1 and FSCN1 in breast cancer and their relationship with the clinical pathological features of breast cancer. Methods The protein expressions of Notch1, FSCN1, estrogen receptor (ER), progesterone receptor (PR), CerbB-2, and ki-67 of 100 breast cancer tissue samples and 30 adjacent normal breast tissue samples were detected by the immunohistochemical method. The mRNA expressions of Notch1 and FSCN1 of 60 fresh breast cancer tissue samples and corresponding adjacent tissue samples were detected by the RT-PCR. Results For 100 breast cancer tissue samples and 30 adjacent normal breast tissue samples, the positive rates of Notch1 were 79.0% (79/100) and 33.3% (10/30) and the positive rates of FSCN1 were 68.0% (68/100) and 30.0% (9/30). The positive rates of Notch1 and FSCN1 of breast cancer tissue samples were significantly higher than those of adjacent normal breast tissue samples (P<0.05). The positive expression of Notch1 of breast cancer was not significantly relevant to age, ER, and PR (P>0.05), but was relevant to axillary lymph node metastasis, clinical TNM stage, pathological classification, CerbB-2, and ki-67 (P<0.05). The positive expression of FSCN1 of breast cancer was not relevant to age (P>0.05), but was relevant to axillary lymph node metastasis, clinical TNM stage, pathological classification, ER, PR, CerbB-2, and ki-67 (P<0.05). The protein expressions of Notch1 and FSCN1 were positively related (r=0.225, P<0.05). The results of RT-PCR showed that the expressions of Notch1 and FSCN1 of breast cancer tissue were higher than those of adjacent normal breast cancer tissue and the differences were statistically significant (P<0.05). The mRNA expressions of Notch1 and FSCN1 were positively related (r=0.446, P<0.05). Conclusion The expressions of Notch1 and FSCN1 of breast cancer are high and may promote the occurrence and development of breast cancer and play an important role in the invasion, metastasis, and prognosis of breast cancer.