Objective To construct the decellular artificial dermis that carries nanospheres containing platelet derived growth factor-BB (PDGF-BB) and to explore the feasibility of repairing skin defects of nude mice by the constructed decellular artificial dermis (DAD). Methods Using tissue engineered technologies, nanospheres were made by enveloping PDGF-BB in the copolymer of polylactic acid-glycolic acid (PLGA). Nanospheres were then bonded to DAD. The decellular human dermis which carried nanospheres containing PDGF-BB was transplanted to nude mice (the experiment group, n=10). Nude mice of the control group (n=10) were transplanted by the decellular human dermis which carried nanospheres not containing PDGF-BB. The repair effect of decellular human dermis which carried nanospheres containing PDGF-BB was observed. Expressions of a-SMA and lumican in local tissue were detected by the Real-Time PCR and Western blotting. Results The decellular human dermis which carried nanospheres containing PDGF-BB repaired skin defects more quickly than the decellular human dermis which carried nanospheres not containing PDGF-BB. The difference was statistically significant (P<0.05). Relative expressions of a-SMA and lumican in local tissue increased significantly after transplantation of the decellular human dermis which carried nanospheres containing PDGF-BB. Conclusion The decellular human dermis which carried nanospheres containing PDGF-BB can significantly improve the treatment of skin defects and is possible to be used in clinical applications.