Original article (Basic research)

Expression and biologic effects of microRNA-375 in triple negative breast cancer

  • YANG Chen ,
  • YU Pei ,
  • LI Ji-yu ,
  • et al
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  • 1.Department of Medical Oncology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2.Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 3.Department of Breast and Thyroid Surgery, Provincial Hospital Affilliated to Shandong University, Jinan 250021, China; 4.Department of Chemistry and Radiation Oncology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Online published: 2014-12-02

Supported by

Cultivation Program for Young Teachers in Shanghai Colleges, 2012-046

Abstract

Objective To investigate the expression and biologic effects of microRNA-375 (miRNA-375) of triple negative breast cancer (TNBC). Methods The expression of miRNA-375 of tumor tissue and adjacent normal tissues of 62 patients with TNBC was detected by the Real-time PCR. The relationship of the expression of miRNA-375 and clinicopathological features was analyzed. The expressions of miRNA-375 in breast cancer cell line MDA-MB-231 and breast fibroadenoma cell line MCF-10A were detected. The miRNA-375 precursor molecule was transfected to MDA-MB-231. The variations of the proliferation and invasion of MDA-MB-231 were detected by CCK-8 kit and Transwell test. Results The expression of miRNA-375 of TNBC tissue was significantly lower than that of adjacent normal tissue (P<0.001) and was relevant to the tumor size and axillary lymph node metastasis (P<0.001). Compared to MCF-10A, the expression of miRNA-375 in MDA-MB-231 significantly decreased (P=0.021). After being transfected by the miRNA-375 precursor, the expression of miRNA-375 in MDA-MB-231 significantly increased and the proliferation and invasion abilities decreased. Conclusion The expression of miRNA-375 in TNBC tissue and MDA-MB-231 is down-regulated. And miRNA-375 can inhibit the carcinogenesis and development of TNBC to a certain extent.

Cite this article

YANG Chen , YU Pei , LI Ji-yu , et al . Expression and biologic effects of microRNA-375 in triple negative breast cancer[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2014 , 34(11) : 1586 . DOI: 11.3969/j.issn.1674-8115.2014.11.005

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