Expression and biologic effects of microRNA-375 in triple negative breast cancer
Online published: 2014-12-02
Supported by
Cultivation Program for Young Teachers in Shanghai Colleges, 2012-046
Objective To investigate the expression and biologic effects of microRNA-375 (miRNA-375) of triple negative breast cancer (TNBC). Methods The expression of miRNA-375 of tumor tissue and adjacent normal tissues of 62 patients with TNBC was detected by the Real-time PCR. The relationship of the expression of miRNA-375 and clinicopathological features was analyzed. The expressions of miRNA-375 in breast cancer cell line MDA-MB-231 and breast fibroadenoma cell line MCF-10A were detected. The miRNA-375 precursor molecule was transfected to MDA-MB-231. The variations of the proliferation and invasion of MDA-MB-231 were detected by CCK-8 kit and Transwell test. Results The expression of miRNA-375 of TNBC tissue was significantly lower than that of adjacent normal tissue (P<0.001) and was relevant to the tumor size and axillary lymph node metastasis (P<0.001). Compared to MCF-10A, the expression of miRNA-375 in MDA-MB-231 significantly decreased (P=0.021). After being transfected by the miRNA-375 precursor, the expression of miRNA-375 in MDA-MB-231 significantly increased and the proliferation and invasion abilities decreased. Conclusion The expression of miRNA-375 in TNBC tissue and MDA-MB-231 is down-regulated. And miRNA-375 can inhibit the carcinogenesis and development of TNBC to a certain extent.
Key words: triple negative breast cancer; microRNA-375; invasiveness
YANG Chen , YU Pei , LI Ji-yu , et al . Expression and biologic effects of microRNA-375 in triple negative breast cancer[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2014 , 34(11) : 1586 . DOI: 11.3969/j.issn.1674-8115.2014.11.005
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