Objective To observe the effect of green tea polyphenol extract, (-)-epigallocatechin-3-gallate (EGCG), on anti-angiogenesis. Methods Fresh chicken eggs with normal chicken embryos were randomly divided into the blank control group, PBS group, low dose EGCG group, medium dose EGCG group, and high dose EGCG group after being incubated for 5 d and each group consisted of 7 eggs. Then, 10 μL sample was injected through the air chamber of eggs (EGCG solutions of 100, 1 000, and 10 000 μg/mL were used for the low dose, medium dose, and high dose EGCG groups, respectively). Chick chorioallantoic membrane (CAM) was solidified and cut out after 2 d. The angiogenesis of CAM of each group was photographed recorded and the vascular intersection number (VN) was manually counted. The vessel angiogenesis area (VA) was scanned and analyzed and the ratio of VA and CAM (VA/CAM) was calculated. Results Blood vessels of CAM of the blank control group and PBS group were abundant and thick. With the increase of EGCG concentration, blood vessels were gradually sparse and scattered. VNs of 1 cm×1 cm area of the PBS group, low dose EGCG group, medium dose EGCG group, and high dose EGCG group were 9.5±1.29, 9.8±1.30, 8.2±1.30, and 6.5±0.58, respectively. The differences of the high dose EGCG group and other groups were statistically significant (P<0.05). VA/CAM values of the PBS group, low dose EGCG group, medium dose EGCG group, and high dose EGCG group were 0.24±0.04, 0.18±0.01, 0.14±0.02, and 0.08±0.01, respectively. The differences among groups were statistically significant (P<0.01). Conclusion Green tea polyphenol can inhibit the angiogenesis of CAM with dose-dependent effect.