Original article (Basic research)

Inhibition of green tea polyphenol on angiogenesis of chick chorioallantoic membrance

  • LIU Chang ,
  • SHEN Xiu-hua ,
  • TANG Wen-jing ,
  • et al
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  • 1.Department of Nutrition, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2.College of Software, Nankai University, Tianjin 300071, China

Online published: 2015-01-29

Supported by

National Natural Science Foundation of China, 81102123;Shanghai Undergraduate Innovation Training Program, IAP6125

Abstract

Objective To observe the effect of green tea polyphenol extract, (-)-epigallocatechin-3-gallate (EGCG), on anti-angiogenesis. Methods Fresh chicken eggs with normal chicken embryos were randomly divided into the blank control group, PBS group, low dose EGCG group, medium dose EGCG group, and high dose EGCG group after being incubated for 5 d and each group consisted of 7 eggs. Then, 10 μL sample was injected through the air chamber of eggs (EGCG solutions of 100, 1 000, and 10 000 μg/mL were used for the low dose, medium dose, and high dose EGCG groups, respectively). Chick chorioallantoic membrane (CAM) was solidified and cut out after 2 d. The angiogenesis of CAM of each group was photographed recorded and the vascular intersection number (VN) was manually counted. The vessel angiogenesis area (VA) was scanned and analyzed and the ratio of VA and CAM (VA/CAM) was calculated. Results Blood vessels of CAM of the blank control group and PBS group were abundant and thick. With the increase of EGCG concentration, blood vessels were gradually sparse and scattered. VNs of 1 cm×1 cm area of the PBS group, low dose EGCG group, medium dose EGCG group, and high dose EGCG group were 9.5±1.29, 9.8±1.30, 8.2±1.30, and 6.5±0.58, respectively. The differences of the high dose EGCG group and other groups were statistically significant (P<0.05). VA/CAM values of the PBS group, low dose EGCG group, medium dose EGCG group, and high dose EGCG group were 0.24±0.04, 0.18±0.01, 0.14±0.02, and 0.08±0.01, respectively. The differences among groups were statistically significant (P<0.01). Conclusion Green tea polyphenol can inhibit the angiogenesis of CAM with dose-dependent effect.

Cite this article

LIU Chang , SHEN Xiu-hua , TANG Wen-jing , et al . Inhibition of green tea polyphenol on angiogenesis of chick chorioallantoic membrance[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2015 , 35(1) : 13 . DOI: 11.3969/j.issn.1674-8115.2015.01.003

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