Isolation and molecular epidemiology of ST571 NDM-1 producing Klebsiella pneumoniae
Online published: 2015-03-26
Objective To investigate the molecular epidemiological significance of isolating a New Delhi metallo-β-lactamase-1 (NDM-1) producing Klebsiella pneumoniae (K. pneumoniae) strain. Methods The strain was a carbapenems resistant K. pneumoniae strain isolated from clinical samples. VITEK-2 Compact instrument was used for identification and antibiotic susceptibility test. Expressions of NDM-1 genes and carbapenemases genes were detected by the PCR. The transfer plasmid conjugation experiment and multilocus sequence typing (MLST) were performed. MLST data of NDM-1 producing K. pneumoniae from other areas was collected from PubMed and analyzed by eBURST V3 software and Tree drawing software. Results The isolated strain was highly resistant to many antibiotics including carbpenems and carried the NDM-1 gene. Plasmids that contained the NDM-1 gene were successfully transferred to recipient bacteria by conjugation and the MLST of K. pneumoniae strain was ST571. The MLST data of 283 NDM-1 producing K. pneumoniae strains from 28 countries were collected, including 42 ST strains. The major epidemic strain was CC258 strain (clone complex). Conclusion The ST571 NDM-1 producing K. pneumoniae strain is isolated in China for the first time. The drug resistant gene may be transmitted by plasmids. The molecular typing of NDM-1 producing K. pneumoniae is diverse. CC258 strain is the most epidemic strain. The isolated strain is genetically far from cloned CC258 strain.
LIU Jing-xian , YU Jing , LIU Ying . Isolation and molecular epidemiology of ST571 NDM-1 producing Klebsiella pneumoniae[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2015 , 35(3) : 402 . DOI: 11.3969/j.issn.1674-8115.2015.03.019
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