Original article (Basic research)

Gene detection and genetic characteristic analysis of PER type extended-spectrum β-lactamases in Enterobacteriaceae

  • XIE Lian-yan ,
  • WANG Xiao-li ,
  • ZHANG Fang-fang ,
  • et al
Expand
  • 1.Department of Clinical Microbiology, 2.Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Online published: 2015-04-29

Supported by

National Natural Science Foundation of China, 81472010

Abstract

Objective To investigate genotypes, epidemiological characteristics, and possible  transmit mechanisms of resistance of PER type extended-spectrum β-lactamase in clinical Enterobacteriaceae isolates. Methods A total of 254 clinical isolates of Enterobacteriaceae with resistance to ceftazidime, cefotaxim, and cefepime were collected from June, 2009 to January, 2014 in Ruijin Hospital, Shanghai Jiao Tong University School of Medicine. The blaPER was identified by the PCR and sequencing. Conjugation experiments were performed for blaPER-positive strains and epidemiological analysis was conducted by the pulsed field gel electrophoresis (PFGE). The upstream and downstream sequences of PER gene were amplified by the PCR. Results A total of 14 clinical Enterobacteriaceae isolates carried the blaPER, including PER-1 type and PER-4 type. Two P. mirabilis isolates were successfully transferred by IncA/C conjugative plasmid. Nine isolates of blaPER-positive P. mirabilis showed 7 different PFGE patterns. The most common genetic environment of Enterobacteriaceae was ISCR1-blaPER-gst-abct and the genetic environment of 2 blaPER-positive P. mirabilis were ISPa12-blaPER-gst-like-ISPa13. Conclusion The blaPER was detected among a variety of clinical Enterobacteriaceae isolates. The upstream genetic environment of blaPER is given priority to the genetic environment with ISCR1 element, which may play an important role in the transmission of Chinese blaPER gene.

Cite this article

XIE Lian-yan , WANG Xiao-li , ZHANG Fang-fang , et al . Gene detection and genetic characteristic analysis of PER type extended-spectrum β-lactamases in Enterobacteriaceae[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2015 , 35(4) : 494 . DOI: 11.3969/j.issn.1674-8115.2015.04.006

Outlines

/