Objective  To evaluate the expression of Musashi-1 in endometrioid adenocarcinoma (EAC) and its value for assessing the prognosis. Methods  The mRNA expressions of Musashi-1 of 35 EAC samples and 15 normal endometrium samples were detected by real-time PCR. The protein expressions of Musashi-1 of 148 EAC samples and 20 normal endometrium samples were detected by immunohistochemistry. The correlation between Musashi-1 expression and survival rate of patients was analyzed. The value of Musashi-1 expression for predicting the prognosis of EAC was evaluated according to clinicopathological indexes. Results  The mRNA expression of Musashi-1 of EAC tissues was significantly higher than that of normal endometrium tissues (P<0.01). Musashi-1 expression correlated with tumor stage, grade, and vascular invasion (χ²=6.276, P<0.01; χ²=26.957 P<0.01； χ²=9.091, P<0.01). The survival rate of patients with positive expression of Musashi-1 was significantly lower than that of patients with negative expression (HR=2.073, P<0.01). Conclusion  The Musashi-1 expression of EAC is high and negatively correlates with the prognosis of EAC. Musashi-1 can be used as an independent factor for predicting the prognosis of EAC and may be a new target for cancer treatment.