Objective  To investigate the feasibility, reliability and validity of applying the Chinese version of German Questionnaire for the Subjective Life Quality of Inhabitants in Nursing Homes to the evaluation of the subjective life quality of inhabitants in Shanghai nursing homes. Methods  Stratified cluster sampling was adopted and a total of 330 inhabitants of nursing homes were surveyed by the questionnaire. Among them, 56 respondents were resurveyed. The feasibility, reliability of internal consistence and retest, structural validity, standard correlation validity, and discriminant validity of the questionnaire were evaluated. Results  The mean time for respondents to complete the questionnaire was (24.14±9.53) min (<30 min) and the feasibility was satisfactory. The reliability coefficient of internal consistence (Cronbach’α) was 0.776 and Cronbach’α coefficients of all domains were >0.7, which indicated that the reliability of internal consistence was satisfactory and could be used for the survey of life quality. The test-retest correlation coefficient of total score was 0.698 (P<0.001). In confirmatory factor analysis, root mean square error of approximation (RMSEA) was 0.080 and comparative fit index (CFI) was 0.601 (χ²=2 931.5，P<0.001), which meant that the results of survey met the purpose of questionnaire and the structural validity was satisfactory. The life satisfaction evaluated by respondents was adopted as the criterion and the correlation coefficient between the total score of life quality and life satisfaction was 0.234 7 (P<0.001), indicating the total score of life quality positively correlated with life satisfaction and the standard correlation validity was acceptable. The results of nonparametric test showed that different moods significantly affected the total score of life quality of respondents (χ²=7.961，P<0.001). Cohen’s d values of any two adjacent levels were greater than 0.5, which showed that the discriminant validity was satisfactory. Conclusion  The feasibility, reliability of internal consistence and retest, structural validity, and discriminant validity of Chinese version of German Questionnaire for the Subjective Life Quality of Inhabitants in Nursing Homes are satisfactory and the standard correlation validity is acceptable. However, further research in more areas should be conducted before it can be widely used.