Objective  To provide a new experimental method for studying the mechanisms of epileptic network by integrating the multi-channel in vivo recording techniques and the establishment of epilepsy model. Methods  Multi-channel in vivo recording techniques were adopted to simultaneously record neural activities of the basolateral amygdala, cornu ammonis 1 of hippocampus, primary somatosensory cortex, and mediodorsal thalamus of amygdala-kindling mice. Meanwhile, behaviors of mice were also observed and recorded. Results  The afterdischarge was recorded in four recording regions for epilepsy from grade 1 to 5 and the duration of afterdischarge increased with the grade of epilepsy. The incidence and development of epilepsy accompanied dynamic changes of frequency-specific electrical signals. Conclusion  This experimental method may be a valuable method for study the network mechanism of the ictogenesis and epileptogenesis.