Objective  To investigate the current status of occupational value of medical staff and analyze the influence rules of demographic variables towards the occupational value. Methods  Questionnaire was designed according to the work value inventory (WVI) and characteristics of Chinese medical staff. Then 516 staff of a new tertiary grade A hospital in Shanghai were surveyed. The independent sample t test or univariate variance analysis was performed to determine the correlations between the occupational value and demographic variables, including gender, age, position, title, education level, and marital status. Results  The differences of occupational value of medical staff with different genders, education levels, and marital statuses were not statistically significant (P>0.05), but the differences of occupational value of medical staff with different ages, positions, and titles were statistically significant (P<0.05). Conclusion  Demographic variables have certain influence on the occupational value of medical staff. To satisfy the needs of medical staff with different background can maintain the work enthusiasm, improve the work performance, and enhance the quality of medical service.