Effects of singlestranded DNA binding protein 2 on trophoblastic differentiation in mouse embryonic stem cells
Online published: 2017-06-02
Supported by
National Natural Science Foundation of China, 31271456
Objective To identify novel genes promoting trophoblastic differentiation in mouse embryonic stem (ES) cells. Methods Inducing conditions for trophoblastic differentiation in mouse ES cells were optimized. Combined with data analysis, significantly upregulated genes during this differentiation process were identified and singlestrandedDNA binding protein 2 (Ssbp2) was discovered as a candidate gene involved in trophoblastic differentiation. Changes in Ssbp2 expression during the process of trophoblastic differentiation in mouse ES cells, as well as expression patterns of Ssbp2 in three cell types corresponding to early mouse embryos, were detected by qRT-PCR. Then Ssbp2 genes were cloned and overexpressed in mouse ES cells. Cell morphology was observed and whether Ssbp2 genes could promote trophoblastic differentiation in mouse ES cells was detected. Results Through trials with different combinations, we found serumfree mouse TS cell basic medium with BMP4 and bFGF could not only induce significant upregulation of trophoblast markers in mouse ES cells, but also efficiently repress the up-regulation of mesoderm markers. Expression pattern analysis showed that the expression level of Ssbp2 was significantly up-regulated during the process of inducing trophoblastic differentiation in mouse ES cells and Ssbp2 expression was high in mouse trophoblastic TS cells. After overexpression of Ssbp2 in mouse ES cells, ES cells were partially differentiated and the up-regulation in trophoblastic markers was most significant. Conclusion Ssbp2 can promote trophoblastic differentiation in mouse ES cells.
Key words: embryonic stem cell; trophoblast; differentiation; Ssbp2
LIU Jifeng , LI Hui . Effects of singlestranded DNA binding protein 2 on trophoblastic differentiation in mouse embryonic stem cells[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2016 , 36(3) : 313 . DOI: 10.3969/j.issn.16748115.2016.03.001
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