Original article (Basic research)

Effects of miR-506 on the proliferation and metastasis in gastric cancer cells

  • LIN Zi-long ,
  • ZHOU Quan ,
  • REN Jia ,
  • LI Jian-fang ,
  • YU Bei-qin ,
  • SU Li-ping ,
  • XU Min-juan ,
  • LIU Bing-ya
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  • 1.Shanghai Center for Systems Biomedicine, Ministry of Education Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China; 2.Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025,China

Online published: 2016-05-26

Supported by

National Natural Science foundation of China, 91529302; National Key Technology Research and Development Program of the Ministry of Science and Technology of China, 2014BAI09B03

Abstract

Objective To investigate the effects of miR-506 on inhibiting the proliferation and metastasis in gastric cancer cells. Methods The miR-506 expressions in five gastric cancer cell lines (MKN-45, SGC-7901, BGC-823, NCI-N87, and AGS) were measured with real-time fluorescent quantitative PCR. The immortalized human normal gastric mucosa epithelial cell line GES-1 was served as control. MiR-506 mimics and anti-miR-506 were transiently transfected into SGC-7901 cell line to up-regulate and down-regulate the miR-506 expression with liposome transfection method. Changes in the proliferation of SGC-7901 were observed with CCK-8, while cell migration and invasion abilities were observed with wound healing assay and Transwell migration and invasion assay. Results The expressions of miR-506 in five gastric cancer cell lines were significantly lower as compared with normal gastric mucosa epithelial cell line GES-1 (P<0.05). MiR-506 over-expression could significantly inhibit the proliferation, migration, and invasion abilities in SGC-7901 cells. Compared with negative controls, the difference was statistically significant (P<0.01).The inhibition of miR-506 expression could promote cell proliferation, migration, and invasion abilities (P<0.01). Conclusion The expression of miR-506 significantly decreases in gastric cancer cell lines. Up-regulation of miR-506 can inhibit proliferation, migration, and invasion abilities in gastric cancer cells, while down-regulation of miR-506 has the opposite effects.

Cite this article

LIN Zi-long , ZHOU Quan , REN Jia , LI Jian-fang , YU Bei-qin , SU Li-ping , XU Min-juan , LIU Bing-ya . Effects of miR-506 on the proliferation and metastasis in gastric cancer cells[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2016 , 36(5) : 613 . DOI: 10.3969/j.issn.1674-8115.2016.05.001

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