Objective·To compare the accuracy and consistency between Lenstar LS900 and Tomey EM-3000 for measuring central corneal thickness after vitrectomy. Methods·Fifty-four patients (55 aphakic eyes) who had undergone post-vitrectomy for at least three months were assigned to three groups based on the axial length, i.e. group A (axial length≥26 mm), group B (axial length between 24 mm and 26 mm), and group C (axial length＜24 mm ). The patients were also assigned to the silicone oil-filled eye group (26 eyes) and the non-silicone oil-filled eye group (29 eyes), and then each group was assigned to three groups according to the axial length. The central corneal thickness was measured using Lenstar LS900 and Tomey EM-3000 analyzer and the data were statistically analyzed. Results·The mean central corneal thickness values measured by Lenstar LS900 and Tomey EM-3000 were (550.44±32.03) μm and (547.58±34.29) μm, respectively and the difference was statistically significant (P<0.01). But the differences in mean central corneal thickness values between the two methods in group A, group B, or group C were not statistically significant (P>0.05). The differences in mean central corneal thickness values between the two methods in the silicone oil-filled eye group or the non-silicone oil-filled eye group were not statistically significant (P>0.05). Only the differences in mean central corneal thickness values between the two methods in group A of the non-silicone oil-filled eye group were statistically significant (P<0.05). Mean central corneal thickness values measured by the two methods were linearly correlated (r=0.957, P<0.01). The width of the 95% limit of agreement ranged from -16.77 μm to 22.47 μm, containing 92.7% (51/55) measurements. Conclusion·The central corneal thickness measured by Lenstar LS900 is slightly larger than that measured by Tomey EM-3000. The two methods are positively correlated and consistent, and both of them can be used to measure the central corneal thickness after vitrectomy.