Journal of Shanghai Jiao Tong University (Medical Science) >

2017 , Vol. 37 >Issue 2: 190

DOI: https://doi.org/10.3969/j.issn.1674-8115.2017.02.011

Original article (Basic research)

Preparation of liposomal pemetrexed and its effects on breast cancer

  • BAI Fang ,
  • CHEN Ting ,
  • LIU Yan ,
  • WU Ke-jin ,
  • CHEN Qing ,
  • LU Yun-shu ,
  • GE Mei-xin
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  • 1. Department of General Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2. Department of Pharmacy, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 3. Department of Breast Surgery, Obstetrics and Gynaecology Hospital, Fudan University, Shanghai 200011, China

Online published: 2017-02-28

Supported by

Foundation of Shanghai Municipal Health and Family Planning Commission, 20154Y0077; Special Scientific Fund for Cultivation of Outstanding Young
Teachers in Shanghai Universities, zzjdyx13087; Research Foundation of Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 14YJ25

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Abstract

Objective · To prepare the liposomal pemetrexed and investigate its effects on MCF-7 breast cancer cells in vitro and nude mice bearing MCF-7
xenograft tumors. Methods · Liposomal pemetrexed was prepared by film dispersion method. Inhibition of MCF-7 breast cancer cell lines was evaluated
by CCK-8 method, and anti-tumor effects were investigated on Balb/c nude mice bearing MCF-7 xenograft tumors. Results · Liposomal pemetrexed
inhibited the growth of MCF-7 cells. When the concentrations of pemetrexed were 0.20, 0.40 and 10.00 μg/mL, the cell viability in experiment group
(liposomal pemetrexed) was significantly lower than that in control group (pemetrexed of same concentration gradient), with P values of 0.013, 0.035 and
0.041, respectively. Compared with blank group (same volume of PBS), the volumes and weights of tumors of nude mice in experiment group(liposomal
pemetrexed) and control group (same volume of pemetrexed) were significantly lower, and the volume and weight of tumor in experiment group were
also significantly lower than those in control group (P=0.000). Conclusion · Compared to bulk drug of pemetrexed, liposomal pemetrexed can inhibit the
growth of MCF-7 breast cancer cells and the Balb/c nude mice bearing MCF-7 xenograft tumors.

Key words：  pemetrexed; liposome; breast neoplasm; MCF-7 cell; Balb/c nude mouse

Cite this article

BAI Fang , CHEN Ting , LIU Yan , WU Ke-jin , CHEN Qing , LU Yun-shu , GE Mei-xin . Preparation of liposomal pemetrexed and its effects on breast cancer[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2017 , 37(2) : 190 . DOI: 10.3969/j.issn.1674-8115.2017.02.011

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