Original article (Basic research)

IL-10 promotes ocular neovascularization by regulating macrophages

  • SUI Ai-ling ,
  • SU Ting ,
  • GAO Yu-shuo ,
  • ZHU Yan-ji ,
  • XIE Bing
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  • Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Online published: 2017-03-30

Supported by

National Natural Science Foundation of China,81570853

Abstract

Objective · To investigate the role of interleukin-10 (IL-10) in regulating ocular neovascularization (NV). Methods · Expression of IL-10 was investigated in mice with oxygen-induced retinopathy (OIR) and transgenic mice with VEGF expression in photoreceptors by immunofluorescence, RT-PCR, and Western blotting. Mice deficient in IL-10 were used to test the effect of IL-10 in retinal, sub-retinal, and choroidal NV. Results · In OIR mice and transgenic mice with VEGF expression in photoreceptors, the staining intensity and mRNA expression of IL-10 were increased. Mice deficient in IL-10 showed a significant reduction in ischemia-induced retinal NV, and choroidal NV at rupture sites in Bruch’s membrane. Mice lacking IL-10 showed reduced levels of hypoxia-inducible factor-1α (HIF-1α) and suppression of ischemia-induced expression of VEGF and VEGF receptor 1. Macrophage was regulated and reduced in ischemic retina of mice with IL-10 deficiency. Conclusion · IL-10 stimulates ocular NV through modulation of HIF-1α and its target genes VEGF and VEGF receptor 1. IL-10 promotes ocular NV via macrophage response to retina ischemia.

Cite this article

SUI Ai-ling , SU Ting , GAO Yu-shuo , ZHU Yan-ji , XIE Bing . IL-10 promotes ocular neovascularization by regulating macrophages[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2017 , 37(3) : 278 . DOI: 10.3969/j.issn.1674-8115.2017.03.002

Outlines

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