Journal of Shanghai Jiao Tong University (Medical Science) >

2017 , Vol. 37 >Issue 12: 1616

DOI: https://doi.org/10.3969/j.issn.1674-8115.2017.12.006

Effects of insulin-like peptide 6 on renal fibrosis and calcification in unilateral ureteral obstruction mice#br#

  • GAO Lei ,
  • WU Shi-yong ,
  • JIANG Dan ,
  • WANG Ying ,
  • LI Yong-yong ,
  • WANG De-wei ,
  • CHANG Guang-lei ,
  • ZHANG Peng ,
  • QIN Zheng ,
  • ZHANG Dong-ying
Expand
  • 1. Department of Cardiovascular Medicine, 2. Department of Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

Online published: 2018-01-10

Supported by

National Natural Science Foundation of China, 81570212; Chinese Medicine Science and Technology Project of Chongqing Health and Family Planning Commission, ZY201702073; Postgraduate Research and Innovation Project of Chongqing, CYB15093; Cardiac Rehabilitation and Metabolic Therapy Research Foundation; "Advanced" Research Foundation Project of Cardiovascular Health Institute, China Cardiovascular Health Alliance

Fold

Abstract

Objective · To investigate the effects of insulin-like peptide 6 (Insl6) on renal fibrosis and calcification in unilateral ureteral obstruction (UUO) mice.  Methods · Twenty-four SPF male mice with genotypic background of C57BL/6 were divided into Sham (n=8), UUO+saline (n=8) and UUO+Insl6 (n=8) groups randomly. Mice were sacrificed 10 days after operation and renal tissues of surgical side were obtained. Sirus red staining, Masson staining and alizarin red S staining were used to verify the level of collagen and calcium deposition. TGF-β1 expression was determined by Western blotting. Realtime-PCR was used for determining TGF-β1, BMP2, Col1a1, and Col2a1 mRNA expression.  Results · Compared with sham group, fibrotic area especially collagen Ⅰ, calcium deposition, TGF-β1 protein, and TGF-β1, BMP2, Col1a1, and Col2a1 mRNA expression in UUO+saline group significantly
 increased (all P<0.05). As compared with UUO+saline group, fibrotic area especially collagen Ⅰ, calcium deposition, TGF-β1 protein, and TGF-β1,
 BMP2, Col1a1, and Col2a1 mRNA expression in UUO+Insl6 group significantly decreased (all P<0.05).  Conclusion · Insl6 inhibits UUO-induced renal fibrosis and calcification, which may be related to regulation of TGF-β1, collagen Ⅰ , BMP2 and collagen Ⅱ expression levels.

Key words： renal fibrosis; renal calcification; unilateral ureteral obstruction; insulin-like peptide 6

Cite this article

GAO Lei , WU Shi-yong , JIANG Dan , WANG Ying , LI Yong-yong , WANG De-wei , CHANG Guang-lei , ZHANG Peng , QIN Zheng , ZHANG Dong-ying . Effects of insulin-like peptide 6 on renal fibrosis and calcification in unilateral ureteral obstruction mice#br#[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2017 , 37(12) : 1616 . DOI: 10.3969/j.issn.1674-8115.2017.12.006

Options
Outlines

/

〈 〉