Original article (Basic research)

Comparison of ex vivo lung perfusion with cold storage in rat donor lung preservation

  • PAN Xu-feng1* ,
  • LI Jian2* ,
  • PAN Yan3 ,
  • GU Chang1 ,
  • GUO Zhen2 ,
  • YANG Jun1
Expand
  • 1. Department of Thoracic Surgery, 2. Department of Extracorporeal Circulation, 3. Department of Pharmacy, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, China

Online published: 2018-03-09

Supported by

Shanghai Municipal Commission of Health and Family Planning Project for Young Scientists, 20144Y0169

Abstract

Objective · To establish stable rat ex vivo lung perfusion (EVLP) model and compare the effect of preservation between EVLP group and cold
storage group. Methods · Eight SD rats were reconditioned with EVLP for 4 h and 8 SD rats were under 3-h cold storage and 1-h EVLP. The biological
index, wet-to-dry weight ratio and concentration of Evans blue were analyzed between two groups. Results · Wet-to-dry weight ratio of lung tissue in
EVLP group was significantly lower (5.08±0.88 vs 6.09±0.48, P=0.012); Oxygenation index in EVLP group was significantly higher [(337.9±35.5) mmHg
vs (300.5±21.6) mmHg, P=0.023]; Concentration of Evans blue in EVLP group was significantly lower [(36.5±20.3) μg/mL vs (65.0±29.9) μg/mL,
P=0.043]. Conclusion · EVLP as an alternative to traditional cold storage technique can effectively alleviate the lung damage.

Cite this article

PAN Xu-feng1* , LI Jian2* , PAN Yan3 , GU Chang1 , GUO Zhen2 , YANG Jun1 . Comparison of ex vivo lung perfusion with cold storage in rat donor lung preservation[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2018 , 38(2) : 151 . DOI: 10.3969/j.issn.1674-8115.2018.02.007

Outlines

/