] Objective · To prepare a bacterial outer membrane vesicle (OMV) coated poly (lactic-co-glycolic acid) copolymer (PLGA) nanoparticle
loaded with ovalbumin (OVA) and evaluate its intranasal immune effect in mice. Methods · OMV was prepared by ultrafiltration concentration method.
OVA loaded PLGA nanoparticle (NP) was prepared by emulsion-solvent evaporation method. OMV coated PLGA nanoparticle (OMV-PLGA NP) loaded
with OVA was prepared by extrusion method and characterized. BALB/c mice were intranasally immunized and specific sIgA levels in nasal wash,
jejunum and fecal pellet were determined by ELISA. Results · Size of OVA loaded OMV-PLGA NP was (234.4±22.9) nm. The shell-core structure of
OVA loaded OMV-PLGA NP was proved by transmission electron microscope. After 14 d of administration, sIgA antibody levels in nasal wash, jejunum
and fecal pellet of OVA loaded OMV-PLGA NP treated group were the highest in all treated groups. Compared with the group treated with OMV and
OVA, OVA-specific sIgA antibody level in nasal wash, jejunum and fecal pellet of OVA loaded OMV-PLGA NP treated group was increased 1.6, 2.1 and
1.7 times, respectively. Compared with the group treated with OMV and OVA, OMV-specific sIgA antibody level in nasal wash, jejunum and fecal pellet
of OVA loaded OMV-PLGA NP treated group was all increased 1.5 times. Conclusion · This novel nanoparticle drug delivery system can simultaneously
delivery OVA and OMV to antigen presenting cells, resulting in stronger mucosal immune response in mice.