Objective · To explore the effect of intrauterine high-nutrition on cytokine in umbilical cord blood-derived monocytes and nature killer (NK) cells. Methods · Samples of umbilical cord blood were collected macrosomia (n10) and infants with normal birthweight (n18). Monocytes and NK cells were obtainedmagnetic sorting. Purified monocytes were stimulatedlipopolysaccharide (LPS), while purified NK cells were stimulatedphorbol myristate acetate (PMA) and ionomycin (ION). The of cytokines was detectedreal-time PCR and ELISA. Results · There was no significant difference in cytokine in the monocytes treated with PBS between macrosomia and infants with normal birthweight. However, dampened responses following LPS stimulation were observed in macrosomia group, including the mRNA and protein of IL-1α, IL-1β, TNF-α, IL-10 and CCL5. Moreover, mRNA and protein of TNF-α were significantly higher in NK cells macrosomia group, while no significant difference in IFN-γ was found. Conclusion · Intrauterine high-nutrition might result in dampened response in monocytes following LPS stimulation and exaggerated of TNF-α in NK cells.