Objective · To investigate the effect of 3-methyladenine (3-MA) on autophagy, migration and invasion of epithelial ovarian cancer (EOC) cells under hypoxia. Methods · Different concentrations of 3-MA were used to treat EOC cells in hypoxic environment. The of autophagy-associated protein LC3 was detectedWestern blotting. The autophagosome formation was observedtransmission electron microscopy. The proliferation, adhesion, migration and invasion of EOC cells were determinedthiazole blue colorimetry, adhesion test, Transwell migration test and Matrigel invasion test, respectively. Results · 3-MA was able to inhibit the increase of LC3-Ⅱ and autophagosome formation inducedhypoxia. In hypoxic environment, the survival rate of EOC cells was significantly decreased5.00 mmol/L 3-MA for 24 h (P0.000). The adhesion ability of EOC cells was significantly decreased2.50 mmol/L 3-MA for 6 h (P0.007). 1.25 mmol/L and 2.50 mmol/L 3-MA could inhibit the migration and invasion of EOC cells in hypoxic environment (P<0.01). Conclusion · 3-MA can inhibit the autophagy, migration and invasion of EOC cells in hypoxic environment.