Original article (Basic research)

A preliminary study on the impact of Notch1 on RANKL/RANK system in osteoclast via RAW264.7 cells

  • WANG Jun1* ,
  • LIANG Jing1* ,
  • HE Yi-feng2 ,
  • 3
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  • 1. Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2. Department of Radiology, Shanghai First Peoples Hospital, Shanghai Jiao Tong University, Shanghai 200080, China; 3. Department of Radiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Online published: 2019-01-27

Abstract

Objective · To investigate the crosstalk between receptor activator of nuclear factor-κB ligand (RANKL) / receptor activator of nuclear factorκB (RANK) system and Notch signaling pathways. Methods · Moosteoclast precursor cell line RAW264.7 was cultured and stimulatedRANKL (35 ng/L). Real-time PCR and Western blotting analysis were used to determine the s of Notch signaling molecules in RANKL-stimulated RAW264.7 cells. Small interfering RNA (siRNA) transfection was used to inhibit the of Notch1, Dll1 and Dll3 in Notch signaling pathways, then the mRNA and protein s of Rank were detected using real-time PCR and Western blotting analysis, respectively. Results · The s of Notch1, Jagged1 and Jagged2 were down-regulated while the of Dll4 was up-regulated after RANKL stimulation. The stimulation also inhibited the of Hes1 and Hey1. After siRNA transfection, the mRNA s of Notch1, Dll1 and Dll3 were suppressed71% (P0.000), 53% (P0.015) and 70% (P0.000), respectively. The mRNA and protein s of Rank were up-regulated after Notch1 inhibition (P0.003, P0.000). Conclusion · The data infers that the impact of Notch1 on RANKL/RANK system might play a key role in bone resorption conductedosteoclast.

Cite this article

WANG Jun1* , LIANG Jing1* , HE Yi-feng2 , 3 . A preliminary study on the impact of Notch1 on RANKL/RANK system in osteoclast via RAW264.7 cells[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2018 , 38(12) : 1440 . DOI: 10.3969/j.issn.1674-8115.2018.12.008

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