Original article (Basic research)

Comparison of the phagocytosis and inflammatory response of murine macrophages infectedLeptospirastrains 56606v and 56606a

  • FAN Xia1 ,
  • XIA Bi-li2 ,
  • Lü Lin1 ,
  • XU Meng-sha3 ,
  • LI Jia-yin1 ,
  • HE Ping1
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  • 1. Department of Immunology and Microbiology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China; 2. Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China; 3. Taizhou Enze Medical Center, Zhejiang Province, Taizhou 318050, China

Online published: 2019-02-27

Supported by

National Natural Science Foundation of China, 81471908

Abstract

Objective · To compare the response and consequence of momacrophages infectedLeptospira interrogans 56606v and 56606a, and explore the mechanisms of pathogenic Leptospira to cadisease. Methods · Peritoneal macrophages of C57BL/6 mice were infectedpathogenic Leptospira 56606v and non-pathogenic Leptospira 56606a respectively. Immunofluorescence staining was performed to observe phagocytosis and clearance of Leptospira after infection, and realtime-PCR was used to determine cytokine production of macrophages. Results · After 72-hour infection, strain 56606v exhibited a lower phagocytic rate but survived after incubation with peritoneal macrophages compared with strain 56606a, which showed a higher phagocytic rate but was cleared at that time point. Additionally, cytokine production of macrophages incubated with 56606v was lower than that with 56606a. Conclusion · Leptospira interrogans strain 56606v can survive in macrophages, which may contribute to evasion phagocytic clearance and lead to disease, while strain 56606a can be cleared, which implicates a lower pathogenicity.

Cite this article

FAN Xia1 , XIA Bi-li2 , Lü Lin1 , XU Meng-sha3 , LI Jia-yin1 , HE Ping1 . Comparison of the phagocytosis and inflammatory response of murine macrophages infectedLeptospirastrains 56606v and 56606a[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2019 , 39(1) : 16 . DOI: 10.3969/j.issn.1674-8115.2019.01.004

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