Journal of Shanghai Jiao Tong University (Medical Science) >

2019 , Vol. 39 >Issue 4: 353

DOI: https://doi.org/10.3969/j.issn.1674-8115.2019.04.004

Original article (Basic research)

Generation and phenotypic analysis of Fbxo22knockout mice

  • ZHANG Hui-lin ,
  • ZHU Xiao-na ,
  • YANG Shuo ,
  • LIU Meng-di ,
  • ZHU Di ,
  • YU Yun
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  • Center of Molecular Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Online published: 2019-05-23

Supported by

National Natural Science Foundation of China, 81772936; Foundation of the International Peace Maternity & Child Health Hospital of China, GFY5809

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Abstract

Objective · To establish the Fbxo22 knockout momodel and study the biological function of FBXO22. Methods · The Fbxo22 knockout mice were generatedCRISPR-Cas9 technology. The number, appearance, weight of different embryos and mice were measured. Meanwhile, the food intake and survival of Fbxo22-/-mice were analyzed. Results · Although the Fbxo22-/-embryos were present at approximately Mendelian ratios on embryonic day 17.5/18.5, most of them died within 48 hours of birth. Furthermore, those surviving Fbxo22-/- mice showed reduced body size and food intake and decreased life span. Conclusion · FBXO22 is an important, albeit not essential, protein for early postnatal survival and normal development. [Key words]FBXO22; CRISPR-Cas9 technology; early postnatal lethality

Key words： FBXO22; CRISPR-Cas9 technology; early postnatal lethality

Cite this article

ZHANG Hui-lin , ZHU Xiao-na , YANG Shuo , LIU Meng-di , ZHU Di , YU Yun . Generation and phenotypic analysis of Fbxo22knockout mice[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2019 , 39(4) : 353 . DOI: 10.3969/j.issn.1674-8115.2019.04.004

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