Journal of Shanghai Jiao Tong University (Medical Science) >

2019 , Vol. 39 >Issue 8: 843

DOI: https://doi.org/10.3969/j.issn.1674-8115.2019.08.006

Original article (Basic research)

Promotion of 640 nm red light on keratinocyte migration via CD26

  • ZHANG Wen ,
  • FU Xiu-jun ,
  • YAO Min
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  • Department of Plastic and Reconstructive Surgery, Shanghai Ninth Peoples Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Online published: 2019-09-23

Supported by

National Natural Science Foundation of China, 81272113

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Abstract

Objective · To investigate the mechanism of 640 nm red light promoting keratinocyte migration preliminarily. Methods · Human keratinocytes were cultured in vitro and assigned to 4 groups according to different interventions, i.e. control group, sitagliptin group (CD26 inhibited), 640 nm red light group (8 J/cm2) and 640 nm red light+sitagliptin group. The levels of CD26 mRNA and protein in keratinocytes were measured with realtime-PCR and Western blotting, respectively. Cell migration was observed with Transwell assay and scratch assay. Results · The level of CD26 mRNA and the of CD26 protein in 640 nm red light group obviously increased compared with control group, while those of sitagliptin group significantly decreased (all P<0.05). Migration ability of keratinocytes increased in 640 nm red light group and decreased in sitagliptin group observedTranswell assay and scratch assay. Conclusion · The of CD26 in keratinocytes is promotedthe 640 nm red light, which can accelerate keratinocyte migration to facilitate re-epithelialization.

Key words： 640 nm red light; CD26; keratinocyte; wound healing; re-epithelialization

Cite this article

ZHANG Wen , FU Xiu-jun , YAO Min . Promotion of 640 nm red light on keratinocyte migration via CD26[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2019 , 39(8) : 843 . DOI: 10.3969/j.issn.1674-8115.2019.08.006

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