Journal of Shanghai Jiao Tong University (Medical Science) >

2019 , Vol. 39 >Issue 9: 947

DOI: https://doi.org/10.3969/j.issn.1674-8115.2019.09.003

Original article (Basic research)

Fabrication of an antibacterial hydrogel laden with adhesive liposomes for bone repairment

  • XIANG Yi1 ,
  • 2 ,
  • LIU Li-li1 ,
  • 2 ,
  • CUI Wen-guo1 ,
  • 2
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  • 1. Institute of Orthopaedics, Soochow University, Suzhou 215000, China; 2. Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Disease, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University of Medicine, Shanghai 200025, China

Online published: 2019-11-02

Supported by

National Natural Science Foundation of China, 51873107; Shanghai Talent Development Fund, 2018099; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Support, 20171906; Shanghai Jiao Tong University “Medical and Research”Program, ZH2018ZDA04）。

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Abstract

Objective · To construct an antibacterial hydrogel laden with adhesive liposomes (A-LIP-PEG) for bone repair. Methods · Adhesive liposomes (A-LIP) were fabricated based on the Schiff-based reaction between dopamine and the liposome. To prepare the liposome composite hydrogel, the A-LIPs were mixed with thiolated polyethylene glycol (4SH-PEG) which was then crosslinked with Ag+. Bone morphogenetic protein 2 (BMP-2) was loaded in the A-LIPs for its uniform dispersion and sustained release in the hydrogel network. The size distribution and zeta-potential of the A-LIPs were characterized with a particle size analyzer. The morphology of the A-LIPs was observed under transmission electron microscope. Flushing test was employed to examine the viscidity of the A-LIPs, and CCK-8 assay was conducted to demonstrate the biocompatibility of the A-LIP-PEG. Osteogenic activity of the A-LIP-PEG was evaluatedalkaline phosphatase assay and alizarin red S staining in bone marrow mesenchymal stem cells (BMSCs). Results · Strong adhesion toward soft tissue of the A-LIPs was indicatedin vitro study. A-LIP-PEG showed significant inhibition on Staphylococcus aureus. A-LIP-PEG showed superior promotion of osteogenic differentiation to hydrogel laden with liposomes (LIP-PEG), without disrupting cell proliferation. Conclusion · The A-LIP-PEG developed in this study shows nopotential to expand the application of hydrogels to drug delivery, antibacteria and bone repair.

Key words： adhesive liposome; antibacteria; hydrogels; bone repair

Cite this article

XIANG Yi1 , 2 , LIU Li-li1 , 2 , CUI Wen-guo1 , 2 . Fabrication of an antibacterial hydrogel laden with adhesive liposomes for bone repairment[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2019 , 39(9) : 947 . DOI: 10.3969/j.issn.1674-8115.2019.09.003

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