Objective · To reveal the effect of denatured collagen type Ⅰ on the endothelial cell proliferation, migration, and angiogenesis-related proteins . Methods · Human umbilical vein endothelial cells were cultured on the plates coated with normal collagen (normal collagen group), half concentration normal collagen (half collagen group) or denatured collagen (denatured collagen group). CCK-8 assay was performed to test cell proliferation ability two days later. The effect of collagen on cell migration was measuredscratch test. The s of membrane-type 1 matrix metalloproteinase (MT1-MMP) and vascular endothelial growth factor (VEGF) were detectedWestern blotting. The s of angiopoietin 1 (Ang-1) and Ang-2 were measuredELISA. Results · CCK-8 results showed that the proliferation activity of denatured collagen group was significantly higher than that of normal collagen group and half collagen group (PPPConclusion · Denatured collagen type Ⅰ can promote proliferation, migration and MT1-MMP, VEGF, and Ang-2 s in endothelial cells, suggesting that collagen denaturation may play an active role in the process of angiogenesis during wound healing.