Objective · To explore the relationship among perceived stress, personality traits and panic disorder (PD) symptoms, and to test the mediating effect of perceived stress on PD symptoms in different personality traits. Methods · A total of 55 PD patients were selected according to Diagnostic and Statistical Manual of Mental Disorders (Fifth Edition) (DSM-5). The NEO Five-Factor Inventory (NEO-FFI) was used to measure the personality traits. The Panic Disorder Severity Scale (PDSS) was used to measure the PD symptoms severity. The Perceived Stress Scale (PSS) was used to measure the level of the perceived stress. Results · Both the neuroticism, agreeableness subscale scores of NEO-FFI and scores of PSS were significantly correlated with the total scores of PDSS, and the correlation coefficient were r0.379 (P0.004), r-0.420 (P0.001) and r0.330 (P0.014), respectively. The mediating effect analysis showed that perceived stress completely mediated the effect of agreeableness on PD symptoms, and the mediating effect was 32.43%. Conclusion · In personality traits, neuroticism may be positively correlated with PD symptoms, while agreeableness may be negatively correlated with PD symptoms. The perceived stress may play a completely mediating role between agreeableness and PD symptoms.