Journal of Shanghai Jiao Tong University (Medical Science) >

2021 , Vol. 41 >Issue 2: 154 - 158

DOI: https://doi.org/10.3969/j.issn.1674-8115.2021.02.005

Basic research

ATP-sensitive potassium channel negatively regulates hippocampal long-term potentiation maintenance

  • Xiao-lin ZHANG ,
  • Xiao-yun ZHANG ,
  • Gui-qin HE ,
  • Yue KONG ,
  • Zi-kai ZHOU
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  • Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China

Received date: 2020-03-30

  Online published: 2021-02-28

Supported by

Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support(20191835);Program of Shanghai Academic Research Leader(19XD1423300)

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Abstract

Objective

·To investigate the regulatory roles of ATP-sensitive potassium channel (KATP) in the induction and maintenance stages of long-term potentiation (LTP), which is an experimental model for studies of synaptic plasticity and memory.

Methods

·Acute brain slices were prepared from male C57BL/6 mice at 5?6 weeks of age. Electrophysiological recording of the field excitatory postsynaptic potentials was performed at the hippocampal Schaffer collateral-commissural pathway. KATP opener cromakalim was used to activate KATP at basal level, induction and maintenance stages of LTP. Oxygen-glucose deprivation (OGD) was used to induce hypoxia and pathological opening of KATP at the maintenance stage of LTP. Tolbutamide (TOL) was used to block KATP before OGD treatment.

Results

·KATP opening showed no effects on basal synaptic transmission and the induction of LTP, but largely decreased the magnitude of LTP at maintenance stage. OGD rapidly impaired LTP maintenance, which was significantly prevented by TOL pre-treatment.

Conclusion

·KATP negatively regulates hippocampal LTP maintenance in both physiological and pathological conditions.

Key words： ATP-sensitive potassium channel (KATP); long-term potentiation (LTP); synaptic plasticity

Cite this article

Xiao-lin ZHANG , Xiao-yun ZHANG , Gui-qin HE , Yue KONG , Zi-kai ZHOU . ATP-sensitive potassium channel negatively regulates hippocampal long-term potentiation maintenance[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2021 , 41(2) : 154 -158 . DOI: 10.3969/j.issn.1674-8115.2021.02.005

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