Journal of Shanghai Jiao Tong University (Medical Science) >

2021 , Vol. 41 >Issue 8: 1009 - 1016

DOI: https://doi.org/10.3969/j.issn.1674-8115.2021.08.003

Basic research

Expression of protein arginine methyltransferase 5 in lung cancer and its mechanism of promoting lung cancer

  • Bing-qian ZHOU ,
  • Li HAN ,
  • Zhe-yi CHEN ,
  • Shi-yu CHEN ,
  • Ying-xia ZHENG
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  • Department of Laboratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200082, China
ZHENG Ying-xia, E-mail: combi3230@163.com.

Online published: 2021-08-13

Supported by

National Natural Science Foundation of China(81571525);Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support(20161315)

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Abstract

Objective

·To investigate the expression of protein arginine methyltransferase 5 (PRMT5) in lung cancer and its effect on proliferation, migration and invasion of lung cancer cells.

Methods

· Immunohistochemistry was used to detect the expression of PRMT5 in 73 pairs of lung cancer tissues and adjacent tissues, and its correlation with clinical characteristics of patients was analyzed. After PRMT5 was down-regulated in lung cancer cell line NCI-H1299, the effects of PRMT5 on cell proliferation, migration and invasion were observed by CCK8 assay and Transwell chamber, and the expressions of E-cadherin, vimentin and transcription factor SNAI1 were detected by Western blotting. The expression of PRMT5 in tumor cell lines and lung cancer tissues was analyzed by using the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) database, respectively, and the relationship between the expression of PRMT5 in tissues and the prognosis of lung cancer patients was further analyzed.

Results

·Immunohistochemistry showed that the expression of PRMT5 in lung cancer tissues was significantly higher than that in adjacent tissues (P=0.000), and the expression of PRMT5 in stage Ⅲ?Ⅳ was higher than that in stage Ⅰ?Ⅱ (P=0.033). After PRMT5 down-regulation, the proliferation, migration and invasion ability of NCI-H1299 cells decreased, the expression of E-cadherin increased and the expression of vimentin and SNAI1 decreased. The lung cancer sequencing data of TCGA database showed that the expression of PRMT5 in lung cancer tissues was significantly higher than that in normal lung tissues, and the lung adenocarcinoma patients and the lung squamous cell carcinoma patients with higher expression of PRMT5 had lower overall survival rate (P=0.005) and relapse-free survival rate (P=0.028), respectively.

Conclusion

· PRMT5 is highly expressed in lung cancer tissues, which can promote the proliferation, migration and invasion of lung cancer cells. High expression of PRMT5 is associated with poor prognosis of lung cancer.

Key words： protein arginine methyltransferase 5 (PRMT5); lung cancer; proliferation; migration; epithelial-mesenchymal transition (EMT)

Cite this article

Bing-qian ZHOU , Li HAN , Zhe-yi CHEN , Shi-yu CHEN , Ying-xia ZHENG . Expression of protein arginine methyltransferase 5 in lung cancer and its mechanism of promoting lung cancer[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2021 , 41(8) : 1009 -1016 . DOI: 10.3969/j.issn.1674-8115.2021.08.003

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