Journal of Shanghai Jiao Tong University (Medical Science) >

2021 , Vol. 41 >Issue 9: 1272 - 1276

DOI: https://doi.org/10.3969/j.issn.1674-8115.2021.09.022

Brief original article

Clinical application and effect of phage on the treatment of pulmonary infection by pan-drug resistant Klebsiella pneumoniae

  • Li-sha LI ,
  • Jian-hui LI ,
  • Bin HE ,
  • Nan-nan WU ,
  • Tong-yu ZHU ,
  • Xiao-kui GUO ,
  • Zheng-hong CHEN
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  • 1.Department of Microbiology, School of Basic Medical Science, Guizhou Medical University, Guiyang 550025, China
    2.Shanghai Institute of Phage, Shanghai Public Health Clinical Center, Shanghai 201508, China
    3.School of Global Health of Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
CHEN Zheng-hong, E-mail: chenzhenghong@gmc.edu.cn.

Received date: 2021-03-10

  Online published: 2021-08-24

Supported by

Natural Science Foundation of Shanghai(17ZR1415900)

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Abstract

Objective

·To explore the therapeutic frequency and effect of phage treatment on pan-drug resistant Klebsiella pneumoniae by aerosolized phage.

Methods

·The patient's sputum was taken 1 h before the start of treatment and every hour during the treatment of pulmonary infection using nebulized inhaled phage. The sputum samples were subjected to bacterial isolation, purification and mass spectrometry identification. The lytic spectrum of different bacterial isolations was compared. And blood samples for bacteria were taken 1 h before the start of the treatment and 2 h and 4 h after the first treatment for bacterial culture and phage volume determination.

Results

·The phage continued to proliferate in vivo for 2 h, and decreased after 3 h. When the second phage treatment was performed after 4 h, the pattern of phage proliferation in vivo was the same as the first time. During the third treatment, the phage was proliferated slightly in vivo. After phage treatment, Klebsiella pneumoniae was still isolated in the patient's sputum. However, the phage cleavage spectrum of the isolations was different, and the patient's clinical symptoms improved.

Conclusion

·Phages can effectively target infectious bacteria and kill them. The clinical symptoms of patient improve. Twice aerosolized phage treatment for the patient with pulmonary infection is enough to achieve the best effect. The isolated bacterial strain is different post phage treatment.

Key words： phage; pan-drug resistant; Klebsiella pneumoniae; therapeutic application

Cite this article

Li-sha LI , Jian-hui LI , Bin HE , Nan-nan WU , Tong-yu ZHU , Xiao-kui GUO , Zheng-hong CHEN . Clinical application and effect of phage on the treatment of pulmonary infection by pan-drug resistant Klebsiella pneumoniae[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2021 , 41(9) : 1272 -1276 . DOI: 10.3969/j.issn.1674-8115.2021.09.022

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