Journal of Shanghai Jiao Tong University (Medical Science) >

2023 , Vol. 43 >Issue 3: 293 - 300

DOI: https://doi.org/10.3969/j.issn.1674-8115.2023.03.004

Basic research

Expression of tetraspanin 1 in breast cancer and its mechanism in promoting the progression of breast cancer

  • Yuan CAO ,
  • Hongxia WANG ,
  • Ying ZHU ,
  • Junjian LI
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  • Department of Oncology Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201600, China
LI Junjian, E-mail: lijunjian1000@hotmail.com.
ZHU Ying, E-mail: zhuy1974@yeah.net

Received date: 2022-11-25

  Accepted date: 2023-03-15

  Online published: 2023-03-28

Supported by

Natural Science Foundation of Shanghai(22ZR1450000)

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Abstract

Objective ·To investigate the expression of tetraspanin 1 (TSPAN1) in breast cancer and its effect on the migration and invasion of breast cancer cells. Methods ·Immunohistochemistry staining (IHC) was used to detect the expression of TSPAN1 in breast cancer tissues and para-tumor tissues from 106 clinical patients, and to analyze its correlation with the clinical characteristics of patients. The Cancer Genome Atlas (TCGA) database was used to analyze the expression of TSPAN1 mRNA in breast cancer tissues and para-tumor tissues, and its correlation with the clinical characteristics of patients. After down-regulation of TSPAN1 in breast cancer cells MDA-MB-231 and SUM159PT, the effects of TSPAN1 on migration and invasion of cells were detected by wound healing assay and transwell assay, and the expression of epithelial-mesenchymal transition (EMT)-related proteins (E-cadherin, N-cadherin and vimentin) were detected by Western blotting. Results ·IHC results showed that the expression of TSPAN1 in breast cancer tissues was higher than that in para-tumor tissues (P=0.000), and the expression of TSPAN1 in total TNM stages, M stages, N stages and pathological grades of breast cancer tissues showed statistically significant differences (all P<0.05). The results of transcriptome sequencing data from TCGA database showed that the expression of TSPAN1 mRNA in breast cancer tissues was higher than that in para-tumor tissues (P=0.000), and its expression in TNM Ⅲ?Ⅳ stages was higher than that in TNMⅠ?Ⅱ stages (P=0.007). After down-regulation of TSPAN1, the migration and invasion ability of MDA-MB-231 and SUM159PT cells were decreased, the expression of E-cadherin was increased and the expressions of N-cadherin and vimentin were decreased (all P<0.05). Conclusion ·TSPAN1 is highly expressed in breast cancer tissues, which can promote the migration and invasion of breast cancer cells.

Key words： breast cancer; tetraspanin 1 (TSPAN1); epithelial-mesenchymal transition (EMT)

Cite this article

Yuan CAO , Hongxia WANG , Ying ZHU , Junjian LI . Expression of tetraspanin 1 in breast cancer and its mechanism in promoting the progression of breast cancer[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2023 , 43(3) : 293 -300 . DOI: 10.3969/j.issn.1674-8115.2023.03.004

References

1 SIEGEL R L, MILLER K D, JEMAL A. Cancer statistics, 2015[J]. CA Cancer J Clin, 2015, 65(1):5-29.
2 STEEG P S. Targeting metastasis[J]. Nat Rev Cancer, 2016, 16(4): 201-218.
3 ZHU R X, GIRES O, ZHU L Q, et al. TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling[J]. Nat Commun, 2019, 10(1): 2863.
4 HUANG Y W, LI J J, DU W Q, et al. Nuclear translocation of the 4-pass transmembrane protein Tspan8[J]. Cell Res, 2021, 31(11): 1218-1221.
5 LU X Q, AN L W, FAN G J, et al. EGFR signaling promotes nuclear translocation of plasma membrane protein TSPAN8 to enhance tumor progression via STAT3-mediated transcription[J]. Cell Res, 2022, 32(4): 359-374.
6 HEMLER M E. Tetraspanin proteins mediate cellular penetration, invasion, and fusion events and define a novel type of membrane microdomain[J]. Annu Rev Cell Dev Biol, 2003, 19: 397-422.
7 CHEN L, YUAN D Y, WANG G L, et al. Clinicopathological significance of expression of Tspan-1, Jab1 and p27 in human hepatocellular carcinoma[J]. J Korean Med Sci, 2010, 25(10): 1438-1442.
8 WANG S S, LIU X H, KHAN A A, et al. miR-216a-mediated upregulation of TSPAN1 contributes to pancreatic cancer progression via transcriptional regulation of ITGA2[J]. Am J Cancer Res, 2020, 10(4): 1115-1129.
9 CHEN L, ZHU Y Y, ZHANG X J, et al. TSPAN1 protein expression: a significant prognostic indicator for patients with colorectal adenocarcinoma[J]. World J Gastroenterol, 2009, 15(18): 2270-2276.
10 SCHOLZ C J, KURZEDER C, KORETZ K, et al. Tspan-1 is a tetraspanin preferentially expressed by mucinous and endometrioid subtypes of human ovarian carcinomas[J]. Cancer Lett, 2009, 275(2): 198-203.
11 WU Y G, CHEN W X, GONG Y F, et al. Tetraspanin 1 (TSPAN1) promotes growth and transferation of breast cancer cells via mediating PI3K/Akt pathway[J]. Bioengineered, 2021, 12(2): 10761-10770.
12 MENKE C H, POHLMANN P R, BACKES A, et al. Tumor size as a surrogate end point for the detection of early breast cancer: a 30-year (1972?2002), single-center experience in southern Brazil[J]. Breast J, 2007, 13(5): 448-456.
13 LOIBL S, POORTMANS P, MORROW M, et al. Breast cancer[J]. Lancet, 2021, 397(10286): 1750-1769.
14 ESTEVA F J, HUBBARD-LUCEY V M, TANG J, et al. Immunotherapy and targeted therapy combinations in metastatic breast cancer[J]. Lancet Oncol, 2019, 20(3): e175-e186.
15 LI J J, CHEN X L, ZHU L Q, et al. SOX9 is a critical regulator of TSPAN8-mediated metastasis in pancreatic cancer[J]. Oncogene, 2021, 40(30): 4884-4893.
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