Objective To investigate the current situation of excellent course website construction of Shanghai Jiaotong University School of Medicine, and explore room for improvement. Methods Two hundred teachers and 1 668 students were randomly selected from Shanghai Jiaotong University School of Medicine, and survey with questionnaires on excellent course website construction of Shanghai Jiaotong University School of Medicine including the purpose of website visit, frequency of website visit, quality of website resource, usability of website, problems in website visit and advice for improvement of website construction was conducted. Results One hundred and fifty-six effective questionnaires were recovered from teachers, with the recovery rate of 78.0%, and 1 489 effective questionnaires were recovered from students, with the recovery rate of 89.3%. 47.4% teachers and 52.9% students visited the websites for 1 to 3 times each month. 78.1% basic medicine teachers thought the website resource could meet the requirement of education, which was higher than clinical medicine teachers (56.5%) (P<0.01). 68.7% junior teachers were satisfied with the quality of website resource, which was higher than senior teachers (47.2%)(P<0.01). There existed inconvenience for the website end users such as unavailability to browse some contents and low illustration speed. Conclusion A self-managed and configurable excellent course website with easy availability should be constructed to effectively serve the medical education.