Effect of different 16S rDNA universal primers on DGGE patterns of periodontal microbial community
Online published: 2011-05-27
Supported by
National Science and Technology Foundation of the Eleventh Tenth-Five Year Plan, 2007BAI18B02;Shanghai Key Discipline Construction Program, S30206;Shanghai Education Committee Foundation, 09YZ78
Objective To preliminarily assess the effect of different 16S rDNA fragments on denaturing gradient gel electrophoresis (DGGE) patterns of periodontal microbial community. Methods Three typical periodontal bacterial strains were selected, plasmid DNA was constructed to get 16S rDNA V3, V3-V5 and V6-V8 region fragments, and sequencing analysis and BLAST identification were performed. The bands of strains were obtained after DGGE isolation and silver nitrate staining of sequenced plasmid DNA. Results DGGE profiles by three universal primers were different. The bands of 16S rDNA V3 region fragments of typical strains were not single, and bands of different strains could not differ from each other. The single band of 16S rDNA V3-V5 and V6-V8 region fragments of each typical strain was found. Conclusion 16S rDNA V3-V5 and V6-V8 region fragments are suitable for periodontal bacteria structure analysis.
ZHOU Yan-bin, SHU Rong, LIU Da-li . Effect of different 16S rDNA universal primers on DGGE patterns of periodontal microbial community[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2011 , 31(5) : 684 . DOI: 10.3969/j.issn.1674-8115.2011.05.034
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