Objective To investigate the effects of selective blockade of cGMP-dependent protein kinase (PKG) on contractility of vascular rings incubated with lipopolysaccharide (LPS) in rats. Methods Thoracic aortic rings of SD rats were obtained, and were randomly divided into control group, LPS group, Nω-Nitro-L-arginine methyl ester (L-NAME) group and DT-2 trifluoroacetate salt group (DT-2 group). The contraction concentration-response curve, maximal tension (Emax) and concentration required for half maximal response (EC50) of vascular rings to phenylephrine (PE) were measured in vitro at different time points (2 h, 3 h and 4 h after incubation with LPS) in each group. Results The contraction concentrationresponse curves of vascular rings after incubation with LPS for different time were significantly lower than those of control group (P<0.05). L-NAME effectively improved the contractility of vascular rings after incubation with LPS for 2 h, 3 h and 4 h, and EC50 and Emax were significantly different from those of LPS group (P<0.05). DT-2 significantly enhanced the contractility of vascular rings after incubation with LPS for 3 h (P<0.01), and EC50 and Emax were significantly different from those of LPS group (P<0.01), while there was no significant difference in contraction concentration-response curves between DT-2 group and LPS group at the time points of 2 h and 4 h （P＞0.05）. Conclusion Selective blockade of PKG can partially recover the contractility of thoracic aortic rings incubated with LPS in rats.