Objective To evaluate the physical and chemical properties of a novel stabilized neutral potential water, and observe its antimicrobial effect on common multidrug resistant pathogens isolated from burn wards. Methods Novel neutral potential water samples freshly prepared (prepared within 1 month) and preserved for 8 months were selected, parameters of effective chlorine content, oxidation-reduction potential (ORP) value and pH value were employed to evaluate the physical and chemical properties, and investigate the effects of long-term preservation on the physical and chemical properties. Colony growth of common multi-drug resistant pathogens (methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii) isolated from burn wards in culture media with novel stabilized neutral potential water was observed by living bacterial counting, and sterilized normal saline was used to substitute neutral potential water as positive control for parallel test. Results The effective chlorine content, ORP value and pH value of novel stabilized neutral potential water samples freshly prepared and preserved for 8 months were 50 to 100 mg/L, >800 mV and 6.0 to 7.0 respectively, and there was no significant difference in physical and chemical properties between freshly prepared sample and that preserved for 8 months. Living bacterial counting revealed that no colony growth was observed in culture media with novel stabilized neutral potential water freshly prepared and preserved for 8 months. Conclusion Novel stabilized neutral potential water has less side effects on skin, stable physical and chemical properties and favorable antimicrobial effect, and can be an ideal disinfectant in burn wards.