Original article (Basic research)

Effects of heart preservation solution containing Pinacidil on donor heart preservation

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  • Department of Cardiac Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China

Online published: 2011-07-27

Supported by

Foundation from the Ministry of Education of China, 20090073110086

Abstract

Objective To observe the effects of heart preservation solution containing different concentrations of Pinacidil on donor heart preservation. Methods Forty SD rats were randomly divided into normal control group, Celsior solution control group, Celsior solution+0.25 mmol/L Pinacidil group, Celsior solution+0.5 mmol/L Pinacidil group and Celsior solution+1 mmol/L Pinacidil group according to different ways of donor heart preservation, with 8 rats in each group. Langendorff model of isolated heart was established, and isolated hearts were preserved in preservation solutions for 6 h, followed by reperfusion for 30 min. The parameters of cardiac function such as left ventricular end-systolic pressure (LVESP), left ventricular development pressure (LVDP), coronary flow (CF), maximum rate of left ventricular pressure rise (dp/dtmax) and minimum rate of left ventricular pressure rise (dp/dtmin), myocardial ultrastructure and myocardiocyte apoptotic index were observed and compared among groups. Results Compared with Celsior solution control group, Celsior solution+0.25 mmol/L Pinacidil group and Celsior solution+1 mmol/L Pinacidil group, the parameters of LVESP, LVDP, CF, dp/dtmax and dp/dtmin significantly increased (P<0.05), the myocardiocyte apoptotic index significantly decreased (P<0.05), and the structures of myocardiocytes and mitochondria were intact in Celsior solution+0.5 mmol/L Pinacidil group. Conclusion Compared with single Celsior heart preservation solution, Celsior heart preservation solution containing 0.5 mmol/L Pinacidil can decrease myocardiocyte apoptosis after donor heart preservation, promote recovery of cardiac function and improve the effects of hypothermic preservation of donor hearts.

Cite this article

LIANG Wei-tao, Vitali Rusinkevich, ZANG Wang-fu, et al . Effects of heart preservation solution containing Pinacidil on donor heart preservation[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2011 , 31(7) : 927 . DOI: 10.3969/j.issn.1674-8115.2011.07.013

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